FBXW8-Dependent Degradation of MRFAP1 in Anaphase Controls Mitotic Cell Death

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Description

Mof4 family associated protein 1 (MRFAP1) is a 14 kDa nuclear protein, which involves in maintaining normal histone modification levels by negatively regulating recruitment of the NuA4 (nucleosome acetyltransferase of H4) histone acetyltransferase complex to chromatin. MRFAP1 has been identified

Mof4 family associated protein 1 (MRFAP1) is a 14 kDa nuclear protein, which involves in maintaining normal histone modification levels by negatively regulating recruitment of the NuA4 (nucleosome acetyltransferase of H4) histone acetyltransferase complex to chromatin. MRFAP1 has been identified as one of the most up-regulated proteins after NEDD8 (neural precursor cell expressed developmentally down- regulated 8) inhibition in multiple human cell lines. However, the biological function of MRFAP1 and the E3 ligase that targets MRFAP1 for destruction remain mysterious. Here we show, by using an immunoprecipitation-based proteomics screen, that MRFAP1 is an interactor of the F-box protein FBXW8. MRFAP1 is degraded by means of the ubiquitin ligase Cul7/FBXW8 during mitotic anaphase-telophase transition and accumulated in mitotic metaphase. Overexpression of FBXW8 increased the polyubiquitination and decreased the stability of MRFAP1, whereas knockdown of FBXW8 prolonged the half-life of MRFAP1. Moreover, forced expression of MRFAP1 in HeLa cells caused growth retardation and genomic instability, leading to severe mitotic cell death. Thus, Cul7/FBXW8-mediated destruction of MRFAP1 is a regulatory component monitoring the anaphase-telophase transition and preventing genomic instability.

Date Created
2017-10-12
Agent

NifH-Harboring Bacterial Community Composition Across an Alaskan Permafrost Thaw Gradient

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Description

Since nitrogen (N) is often limiting in permafrost soils, we investigated the N[subscript 2]-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples taken along a permafrost thaw gradient in an Alaskan boreal

Since nitrogen (N) is often limiting in permafrost soils, we investigated the N[subscript 2]-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples taken along a permafrost thaw gradient in an Alaskan boreal soil. Samples from minimally, moderately and extensively thawed sites were taken to a depth of 79 cm to encompass zones above and below the depth of the water table. NifH reads were translated with frameshift correction and 112,476 sequences were clustered at 5% amino acid dissimilarity resulting in 1,631 OTUs. Sample depth in relation to water table depth was correlated to differences in the NifH sequence classes with those most closely related to group I nifH-harboring Alpha- and Beta-Proteobacteria in higher abundance above water table depth while those related to group III nifH-harboring Delta Proteobacteria more abundant below. The most dominant below water table depth NifH sequences, comprising 1/3 of the total, were distantly related to Verrucomicrobia-Opitutaceae. Overall, these results suggest that permafrost thaw alters the class-level composition of N[subscript 2]-fixing communities in the thawed soil layers and that this distinction corresponds to the depth of the water table. These nifH data were also compared to nifH sequences obtained from a study at an Alaskan taiga site, and to those of other geographically distant, non-permafrost sites. The two Alaska sites were differentiated largely by changes in relative abundances of the same OTUs, whereas the non-Alaska sites were differentiated by the lack of many Alaskan OTUs, and the presence of unique halophilic, sulfate- and iron-reducing taxa in the Alaska sites.

Date Created
2016-11-24
Agent

Robust and efficient medium access despite jamming

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Description
Interference constitutes a major challenge for communication networks operating over a shared medium where availability is imperative. This dissertation studies the problem of designing and analyzing efficient medium access protocols which are robust against strong adversarial jamming. More specifically, four

Interference constitutes a major challenge for communication networks operating over a shared medium where availability is imperative. This dissertation studies the problem of designing and analyzing efficient medium access protocols which are robust against strong adversarial jamming. More specifically, four medium access (MAC) protocols (i.e., JADE, ANTIJAM, COMAC, and SINRMAC) which aim to achieve high throughput despite jamming activities under a variety of network and adversary models are presented. We also propose a self-stabilizing leader election protocol, SELECT, that can effectively elect a leader in the network with the existence of a strong adversary. Our protocols can not only deal with internal interference without the exact knowledge on the number of participants in the network, but they are also robust to unintentional or intentional external interference, e.g., due to co-existing networks or jammers. We model the external interference by a powerful adaptive and/or reactive adversary which can jam a (1 − ε)-portion of the time steps, where 0 < ε ≤ 1 is an arbitrary constant. We allow the adversary to be adaptive and to have complete knowledge of the entire protocol history. Moreover, in case the adversary is also reactive, it uses carrier sensing to make informed decisions to disrupt communications. Among the proposed protocols, JADE, ANTIJAM and COMAC are able to achieve Θ(1)-competitive throughput with the presence of the strong adversary; while SINRMAC is the first attempt to apply SINR model (i.e., Signal to Interference plus Noise Ratio), in robust medium access protocols design; the derived principles are also useful to build applications on top of the MAC layer, and we present SELECT, which is an exemplary study for leader election, which is one of the most fundamental tasks in distributed computing.
Date Created
2012
Agent

The didacticism of Katakiuchi kidan jiraiya monogatari

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Description
My study centers on the novel Katakiuchi Kidan Jiraiya Monogatari (1806-1807) by Kanwatei Onitake (1760-1818). Jiraiya Monogatari was the first literary reading book to be adapted for the kabuki stage. It was also the prototype on which Mizugaki Egao, Kawatake

My study centers on the novel Katakiuchi Kidan Jiraiya Monogatari (1806-1807) by Kanwatei Onitake (1760-1818). Jiraiya Monogatari was the first literary reading book to be adapted for the kabuki stage. It was also the prototype on which Mizugaki Egao, Kawatake Mokuami, Makino Shouzou; and others based their bound picture books, kabuki, and films. The tale is composed of two revenge incidents, both of which have the same structural framework and are didactic in tone. In my study, I analyze the two revenge incidents by examining their narrative structures. Each incident has the same three-act structure: setup, confrontation, and resolution. The setup of each revenge incident introduces the main characters and their relationships and establishes the dramatic vehicle, which is an unexpected incident that sets the revenge in motion. The confrontation contains myriad non-linear inserts, plot twists, and reversals of fortune, all of which have the effect of a narrative delay. This prolongation of the outcome of a simple revenge plot allows readers the necessary space in which they can form their own judgments regarding good and evil and consider karmic cause and effect. The resolution, including the climax as well as the ending of the revenge, demonstrates the didactic notion of punishing evil and karmic effect. The two revenge incidents embody two rules, kanzen chouaku and inga, which together highlight the didacticism of Jiraiya monogatari.
Date Created
2012
Agent