The Karoon River Basin, with an area of about 67,000 km2, is located in the southern part of Iran and has a complex mountainous terrain. No comprehensive study has been done on the spatial and temporal variations of snow cover in this region to date. In this paper, daily snow data of Moderate Resolution Imaging Spectroradiometer MODIS Terra (MOD10A1) and MODIS Aqua (MYD10A1) were examined from 1 January 2003 to 31 December 2015, to analyze snow cover variations. Due to difficulties created by cloud cover effects, it was crucial to reduce cloud contamination in the daily time series. Therefore, two common cloud removal methods were applied on the daily data. The results suggested that in winter nearly 43% of the Basin’s area experienced a negative trend, while only 1.4% of the Basin had a positive trend for snow-covered days (SCD); trends in fall and spring were less evident in the data. Using a digital elevation model of the Basin, the trends of SCD in 100 m elevation intervals were calculated, indicating a significant positive trend in SCD during the fall season above 3500 m.

While there is a popular perception that Canada is a water-rich country, the Saskatchewan River basin (SRB) in Western Canada exemplifies the multiple threats to water security seen worldwide. It is Canada's major food-producing region and home to globally significant natural resource development. The SRB faces current water challenges stemming from (1) a series of extreme events, including major flood and drought events since the turn of the 21st century, (2) full allocation of existing water resources in parts of the basin, (3) rapid population growth and economic development, (4) increasing pollution, and (5) fragmented and overlapping governance that includes the provinces of Alberta, Saskatchewan, and Manitoba, various Federal and First Nations responsibilities, and international boundaries. The interplay of these factors has increased competition for water across economic sectors and among provinces, between upstream and downstream users, between environmental flows and human needs, and among people who hold different values about the meaning, ownership, and use of water. These current challenges are set in a context of significant environmental and societal change, including widespread land modification, rapid urbanization, resource exploitation, climate warming, and deep uncertainties about future water supplies. We use Sivapalan et al.'s (2012) framework of socio-hydrology to argue that the SRB's water security challenges are symptoms of dynamic and complex water systems approaching critical thresholds and tipping points. To Sivapalan et al.'s (2012) emphasis on water cycle dynamics, we add the need for governance mechanisms to manage emergent systems and translational science to link science and policy to the socio-hydrology agenda.

The study presented in this article adds to the body of research on the socio-cultural dimension of sustainable cities by looking at the efforts of the City of Freiburg, Germany to create neighborhoods that acknowledge the importance of the social dimension of sustainable development. The research in this article is centered on evaluating the social responses of living in Freiburg’s two recognized sustainable neighborhoods Rieselfeld and Vauban. The study focuses on the motivational factors that prompted todays residents of the two neighborhoods to move there in the first place, their level of satisfaction living there now, and their perceived social interactions and level of community engagement. Result show that satisfaction with living in a place and reinforcing its assets through social resiliency or livability can result in long-term community staying power. In general, there were few differences in preferences ratings of physical and social assets between the two communities. The levels of importance of social factors contributing to place satisfaction and staying power were not significantly different in both neighbor-hoods. Having a “cluster” of social factors present that were important to residents contributed significantly to place satisfaction. In fact, survey results showed that it was these social factors that were seen as more important to place satisfaction than the physical attributes of sustainable developments.

Capacity dimensioning in production systems is an important task within strategic and tactical production planning which impacts system cost and performance. Traditionally capacity demand at each worksystem is determined from standard operating processes and estimated production flow rates, accounting for a desired level of utilization or required throughput times. However, for distributed production control systems, the flows across multiple possible production paths are not known a priori. In this contribution, we use methods from algorithmic game-theory and traffic-modeling to predict the flows, and hence capacity demand across worksystems, based on the available production paths and desired output rates, assuming non-cooperative agents with global information. We propose an iterative algorithm that converges simultaneously to a feasible capacity distribution and a flow distribution over multiple paths that satisfies Wardrop's first principle. We demonstrate our method on models of real-world production networks.

Background: Virus infections often result in quasispecies of viral strains that can have dramatic impacts on disease outcomes. However, sequencing of viruses to determine strain composition is time consuming and often cost-prohibitive. Rapid, cost-effective methods are needed for accurate measurement of virus diversity to understand virus evolution and can be useful for experimental systems.

Methods: We have developed a novel molecular method for sequence-specific detection of RNA virus genetic variants called Tentacle Probes. The probes are modified molecular beacons that have dramatically improved false positive rates and specificity in routine qPCR. To validate this approach, we have designed Tentacle Probes for two different strains of Lymphocytic Choriomeningitis Virus (LCMV) that differ by only 3 nucleotide substitutions, the parental Armstrong and the more virulent Clone-13 strain. One of these mutations is a missense mutation in the receptor protein GP1 that leads to the Armstrong strain to cause an acute infection and Clone-13 to cause a chronic infection instead. The probes were designed using thermodynamic calculations for hybridization between target or non-target sequences and the probe.

Results: Using this approach, we were able to distinguish these two strains of LCMV individually by a single nucleotide mutation. The assay showed high reproducibility among different concentrations of viral cDNA, as well as high specificity and sensitivity, especially for the Clone-13 Tentacle Probe. Furthermore, in virus mixing experiments we were able to detect less than 10% of Clone-13 cDNA diluted in Armstrong cDNA.

Conclusions: Thus, we have developed a fast, cost-effective approach for identifying Clone-13 strain in a mix of other LCMV strains.

China’s South–North Water Transfer Project (SNWTP) has the potential to transfer as much as 44.8 km³ year^-1 of water from the Yangtze River basin to the Yellow River basin. However, the SNWTP has not been assessed from a sustainability perspective. Thus, in this study we evaluated the SNWTP’s economic, social, and environmental impacts by reviewing the English literature published in journals that are part of the Web of Science database. We then synthesized this literature using a Triple Bottom Line framework of sustainability assessment. Our study has led to three main findings: (1) whether the SNWTP is economically beneficial depends largely on model assumptions, meaning that economic gains at the regional and national level are uncertain; (2) the SNWTP requires the resettlement of hundreds of thousands of people and challenges existing water management institutions, suggesting possible social concerns beyond the short term; and (3) evidently large environmental costs in water-providing areas and uncertain environmental benefits in water-receiving areas together point to an uncertain environmental future for the geographic regions involved. Thus, the overall sustainability of SNWTP is seriously questionable. Although much work has been done studying individual aspects of SNWTP’s sustainability, few studies have utilized the multi-scale, transdisciplinary approaches that such a project demands. To minimize environmental risks, ensure social equity, and sustain economic benefits, we suggest that the project be continuously monitored in all three dimensions, and that integrated sustainability assessments and policy improvements be carried out periodically.

Previous proof-of-concept measurements on single-layer two-dimensional membrane-protein crystals performed at X-ray free-electron lasers (FELs) have demonstrated that the collection of meaningful diffraction patterns, which is not possible at synchrotrons because of radiation-damage issues, is feasible. Here, the results obtained from the analysis of a thousand single-shot, room-temperature X-ray FEL diffraction images from two-dimensional crystals of a bacteriorhodopsin mutant are reported in detail. The high redundancy in the measurements boosts the intensity signal-to-noise ratio, so that the values of the diffracted intensities can be reliably determined down to the detector-edge resolution of 4 Å. The results show that two-dimensional serial crystallography at X-ray FELs is a suitable method to study membrane proteins to near-atomic length scales at ambient temperature. The method presented here can be extended to pump–probe studies of optically triggered structural changes on submillisecond timescales in two-dimensional crystals, which allow functionally relevant large-scale motions that may be quenched in three-dimensional crystals.

We compute one-loop renormalization group equations for non-singlet twist-four operators in QCD. The calculation heavily relies on the light-cone gauge formalism in the momentum fraction space that essentially rephrases the analysis of all two-to-two and two-to-three transition kernels to purely algebraic manipulations both for non- and quasipartonic operators. This is the first brute force calculation of this sector available in the literature. Fourier transforming our findings to the coordinate space, we checked them against available results obtained within a conformal symmetry-based formalism that bypasses explicit diagrammatic calculations and confirmed agreement with the latter.