Anti-gay bias is a prevalent aspect of traditional male gender norms that has negatively impacted the lesbian, gay, bisexual, and transgender (LGBT) community, both in terms of mental and physical health. This study examined how anti-gay bias varies as a function of gender, education level, and field of study. We hypothesized that men will have more bias than women, that seniors in college will be less biased than freshmen in college, and that male students in sub disciplines of engineering that have relatively more women (e.g., biomedical engineering), will be less biased than those in more homogeneous, male-dominated fields (e.g., mechanical engineering). Past research has identified the implications of gender and education level in anti-gay bias; the current study looked to further such research and to investigate possible implications of the impact of settings that have a gender imbalance. A total of 303 undergraduate students from Arizona State University completed an online survey that measured attitudes toward lesbian, gay, bisexual, and transgender individuals and communities. Neither education level nor academic field of study were found to have influenced degree of bias. Limitations and implications of the study are discussed.