This study explored the impact of delivery mode on the diversity of the infant gut microbiome. Four delivery groups were studied: vaginally delivered (VD), planned cesarean section (CSD), emergency cesarean section (ECSD), and vaginally born after previous cesarean (VBAC). The alpha and beta diversity of each delivery group was quantified and compared. Baby ID (uniqueness of each infant) and time (change in microbiome over time) covariates were also included to discover if delivery mode had an impact on the infant microbiome regardless of these covariates. Many significant group-wise and pairwise differences in alpha and beta diversity were found amongst the delivery groups.

This paper examines the multifaceted challenges surrounding college students' nutrition, with a specific focus on Arizona State University (ASU). Examining economic shifts, psychological influences, nutrition knowledge, and body image dynamics, it reveals the profound impact on students' food security and eating behaviors. Despite existing initiatives, persistent gaps in resources remain, necessitating comprehensive interventions to support students effectively. In response, "The Ultimate Health Resource for ASU Students," a website, was developed as an innovative solution. This platform aims to empower students by providing a centralized hub to access vital resources, connect with peers, and discover nutritious recipes. Proposed strategies encompass expanding food pantry offerings, developing mobile applications for nutritional guidance, and fostering partnerships with local organizations. By tackling these challenges head-on and fostering a culture of support, ASU can ensure that all students have equitable access to nutritious food options and the necessary resources to thrive academically and personally. The website serves as a place of empowerment, offering practical solutions and fostering a sense of community among ASU students striving for optimal health and well-being.

The aim of this thesis is to explore and understand how cultural, socio-economic, and political factors intersect to influence and affect societal happiness. We examine this by exploring the discrepancies that exist between the world’s happiest country, Finland, and the world’s 15th happiest nation, the United States. Through our explanation, we find that the cultural differences within nations is the implicit key to success in happiness. More specifically, the differences between femininity, experienced in the Nordic region, and masculinity, experienced in the United States. Social, economic, and political factors are in large part a result of the underlying culture. We see the nuanced ways in which cultural orientations shape social structures and policies which directly impact a society’s well-being. In analysis of the World Happiness Report, we acknowledge the multifaceted nature of happiness metrics, highlighting the importance of inclusive policy-making and responsive governance. Amidst its growing popularity the topic of societal happiness is growing in attention from both the public and policymakers. A collective pursuit of happiness emerges despite the cultural diversity prevalent throughout the world. By examining these complex relationships, we look to offer insight that helps create a deeper understanding of the complex relationship that exists between culture and societal happiness and well-being.

The treatment of melanoma is dependent on what stage the cancer has developed into. Metastatic melanoma is commonly treated with immune checkpoint inhibitors. Unfortunately, not all patients will respond to the treatment as expected. This paper develops important background knowledge on melanoma, how it is treated for each stage, and immune checkpoint inhibitors.

Asphalt is a widely used mixture in the paving and roofing industries and its sales are expected to increase by 57% in the next eight years alone (Mandaokar, 2024). However, it is known to have highly toxic constituents such as benzo[a]pyrene (BaP) and catechol, (National Institute, 1977, Hazard Review, 2000, Neghab et al., 2015, and Rozewski et al., 2023). Lemon juice, which is an inexpensive and easily accessible natural substance that is shown to have health benefits such as increasing insulin sensitivity, aiding with weight loss, and preventing heart disease (Tejpal et al., 2020), may counteract the effects of asphalt. The question of what the biological effects of asphalt, lemon juice, and the combination of the two on adipocytes was tested via computational analysis and experiments. It was predicted that catechol and lemon juice components will show biological effects in adipocytes that could be opposing, additive, or synergistic. A computational analysis involving the docking of fourteen components of asphalt and thirty-five components of lemon juice constituents to a targetome of 7,529 proteins (Ovanessians et al., 2024) suggests that asphalt and lemon juice components have many possible protein targets. Experiments were carried out with 3T3L1 mouse adipocytes to study five different lemon extracts (crude, hexane organic and aqueous, and ether organic and aqueous), and two components of asphalt (catechol and BaP): 1) Thiazolyl Blue Tetrazolium Bromide (MTT) cell viability and toxicity assay, 2) reactive oxygen species fluorescence assay, 3) Nile red staining assay, 4) red oil o staining assay, and a 5) lipidomics analysis on the hexane and ether organic extracts of lemon juice. This study has shown that asphalt components BaP and catechol and lemon juice components combined have the following biological effects on adipocytes: 1) Of the 5 lemon extracts tested, the organic layer of the hexane extract solubilized in DMSO (LE4) decreases differentiation the most. 2) Nile red staining is inhibited by 0.1 mg/mL of LE4, 1 µM BaP, and 20 µM catechol at a similar level. 3) Cell morphology differs between LE4, BaP, and catechol. Future work will include an insulin sensitivity assay to confirm the indicative inhibitory relationship found between lemon juice and asphalt. Expanding upon the lipidomic results of the lemon juices, as well as maximizing the potential of dockings by connecting results with the experiments, may also prove to be useful in future studies.

Objective: This study aims to develop and evaluate a semi-automated workflow using Natural Language Processing (NLP) for sharing positive patient feedback with radiology staff, assessing its efficiency and impact on radiology staff morale. Methods: The HIPAA compliant, institutional review board-waived implementation study was conducted from April 2022 to June 2023 and introduced a Patient Praises program to distribute positive patient feedback to radiology staff collected from patient surveys. The study transitioned from an initial manual workflow to a hybrid process using an NLP model trained on 1,034 annotated comments and validated on 260 holdout reports. The time to generate Patient Praises e-mails were compared between manual and hybrid workflows. Impact of Patient Praises on radiology staff was measured using a 4 question Likert-scale survey and an open text feedback box. Kruskal-Wallis and post-hoc Dunn’s test was performed to evaluate differences in time for different workflows. Results: From April 2022 to June 2023, the radiology department received 10,643 patient surveys. Of those surveys, 95.6% of these surveys contained positive comments, with 9.6% (n = 978) shared as Patient Praises to staff. After implementation of the hybrid workflow in March 2023, 45.8% of Patient Praises were sent through the hybrid workflow and 54.2% were sent manually. Time efficiency analysis on 30-case subsets revealed that the hybrid workflow without edits was the most efficient, taking a median of 0.7 minutes per case. A high proportion of staff found the praises made them feel appreciated (94%) and valued (90%) responding with a 5/5 agreement on 5-point Likert scale responses. Conclusion: A hybrid workflow incorporating NLP significantly improves time efficiency for the Patient Praises program while increasing feelings of acknowledgment and value among staff.

Wetlands produce approximately one third of total global methane emissions and sequester significant amounts of CO2. Salt marshes make up 5% of total wetland area, and therefore are key factors affecting global methane and CO2 emissions. Many marshes are anthropogenically managed either by diking, draining, impoundment, or otherwise restricting tidal exchange. This causes marsh freshening, increases methane emissions, and releases sequestered carbon, all of which can lead to a warming effect on the climate by the greenhouse effect. We studied the formerly impounded Old County salt marsh, found in the Herring River Estuary of Wellfleet, Massachusetts, USA. The USGS Woods Hole Coastal and Marine Science Center installed two eddy covariance flux towers in the Herring River Estuary. These showed that Old County had low methane fluxes (17 nmol/m2/s) compared to another site in the same estuary (112 nmol/m2/s). The question became; why did Old County experience lower methane emissions? We then did a focused study on the Old County location to investigate. We sampled various biogeochemical parameters including pH, salinity, ORP, dissolved Fe, sulfate, chloride, CH4, DOC, and DIC from pore water samples taken June 2022. We also measured extractable iron from a 2015 archived sediment core at Old County. Specifically, we explored the role of Fe in reducing methane through Fe coupled anaerobic oxidation of methane (Fe-AOM). The porewater depth profiles ranged from 10cm to 242 cm in depth and showed Old County as acidic (pH of 3-6.5), mostly fresh, anoxic, highly reducing, and high in dissolved organic carbon (DOC; 2,000-10,000 μM). I divided the depth profiles into two distinct zones, one above 50 cm and one below 50 cm. Overall, Fe-AOM was likely to occur below 50 cm because dissolved Fe increased as CH4 decreased, which is the expected pattern for Fe-AOM. Also, because the ratio of the calculated methane flux (-0.552 nmol m-2 s-1) to the dissolved Fe (0.072 nmol m-2 s-1) was 7.6, which closely matched the 1 to 8 stoichiometry of the Fe-AOM reactions.

HIV/AIDS remains a pressing global health challenge, not only because of its medical complexities but also due to associated stigma and the lack of knowledge of the illness in communities around the world. This thesis analyzed cross-cultural differences and long-term changes in women’s knowledge and stigma around HIV/AIDS in low- and middle-income countries. Using Demographic and Health Survey (DHS) data from 24 countries for knowledge and stigma from 2000-2018, we examined changes in HIV/AIDS knowledge score and stigma score. The findings shed light on the perception of HIV/AIDS knowledge improving while stigma persisted indicative of remaining concerns about the illness amongst women.

The purpose of this experiment was to use real-time quantitative polymerase chain reactions (RT-qPCR) to quantify and analyze differences in expression of U1 snRNA variants across four different human Leukemia cell lines. We found a number of interesting results in the four cell lines. Two variants in particular (vU1.15 and vU1.19), were only expressed in one leukemia cell line each, indicating a potential link between their specific mutations and the type of leukemia associated with the cell lines in which they were expressed. Further research should be conducted to understand these differences and uncover potential clinical applications.