Background: Endometriosis is a condition characterized by the growth of the endometrium, or the tissue that lines the uterus, outside of the uterus, and it is diagnosed through the presence of endometriotic lesions in the pelvic region. The disease is most often associated with abnormal and painful vaginal bleeding. Currently, minimal literature exists concerning the management of endometriosis in low and middle-income countries (LMICs), which may influence the lack of a cultural competent understanding of menstruation in LMICs and, therefore, a lack of evidence-based policies concerning menstruation.

Methods: Social and cultural barriers influencing endometriosis reporting and management in LMICs were examined through a systematic literature review. Online databases yielded a list of relevant studies. Then, use of MAXQDA, a qualitative data analysis software program, helped to extract and code specific text segments from each study that pertain to the research topic. In-context analysis of coded segments revealed the most common trends, which were organized into broader themes.

Results: Findings demonstrated that social and cultural ideas regarding vaginal bleeding influenced the lack of disease reporting and management of endometriosis in LMICs. Socioeconomic challenges include a lack of hygiene and sanitation measures and education regarding menstruation and vaginal bleeding. Also, many diseases associated with the abnormal vaginal bleeding are often disregarded and not prioritized in clinical settings. It also became clear that cultural taboos regarding menstruation and vaginal bleeding often create feelings of anxiety and fear in women and girls throughout communities in LMICs. However, further research is needed to examine the ways in which women in those communities treat symptoms of irregular vaginal bleeding related to endometriosis.

Conclusions: Socioeconomic, gender, and sex-related factors may influence the ways in which endometriosis is reported and treated and may affect the way the related diseases are understood. Evidence-based policies using a culturally competent understanding of abnormal vaginal bleeding in LMICs may help positively affect the reproductive health of women and girls in such areas.

Advancements in both the medical field and public health have substantially minimized the detrimental impact of infectious diseases. Health education and disease prevention remains a vital tool to maintain and propagate this success. In order to determine the relationship between knowledge of disease and reported preventative behavior 180 participants amongst the ASU student population were surveyed about their knowledge and prevention behavior for 10 infectious diseases. Of the 180 participants only 138 were completed surveys and used for analysis. No correlation was found between knowledge or perceived risk and preventative measures within the total sample of 138 respondents, however there was a correlation found within Lyme disease and Giardia exposure to information and prevention. Additionally, a cultural consensus analysis was used to compare the data of 17 US-born and 17 foreign-born participants to analyze patterns of variation and agreement on disease education based on national origins. Cultural consensus analysis showed a strong model of agreement among all participants as well as within the US-born and foreign-born student groups. There was a model of agreement within the questions pertaining to transmission and symptoms. There was not however a model of agreement within treatment questions. The findings suggest that accurate knowledge on infectious diseases may be less impactful on preventative behavior than social expectations.

In the current political moment, sex trafficking is an issues that has gained increased political and media attention. This thesis first analyzes the stories that are told about sex trafficking in policy and the media. Analyzing these stories help us make sense of whose voices, experiences, and needs we listen to, and in relief, whose we do not. Through a case study that evaluates the research, policy work, and advocacy being conducted through the Office of Sex Trafficking Intervention Research at ASU, I first explore how they are dominating the conversation about sex trafficking in Arizona. I offer four critiques on STIR's approach to sex trafficking. First, I critique the language that STIR uses, and the implications of explaining this social issue as sex trafficking instead of survival sex. I then critique the policy and responses around the experiences of LGBTQ youth, and how the theory of dynamic nominalism informs the way we should represent LGBTQ youth in research. Through analyzing specific responses to sex trafficking prevention that STIR offers, such as calling 911, I will explore the need for intersectionality to protect the wellbeing of youth of color. Lastly, through theoretical critiques of neoliberalism, I will explore the ways in which STIR's research, advocacy, and trainings neglect to explore the systems youth must navigate and exist in, and how those systems fail. Through each of these unique critiques, we notice different silences and important considerations that are missing from the work that is dominating the discussion of sex trafficking in the US. Ultimately, this thesis does not argue that we should not care about sex trafficking, but instead argues we need to care more. It explores the ways that acknowledging the complexity and nuance of this great social problem can provide the ability to create meaningful solutions that care for and listen to youth.

Medieval European manuscripts contain a wealth of diverse and imaginative collections of illuminations. The margins are a perfect playground for unique, unusual and fantastic creatures, including an illuminated gradual from 1510 known as the Geese Book. In this paper, I have examined a single illumination from the book in depth. It depicts two female monsters, a wild woman and a female dragon, fighting desperately over a wild child. These two females are not only monsters and fighters, but also mothers. The unusual juxtaposition of the nurturing aspect of maternity and the brutal nature of combat is used to facilitate a discussion about women, violence, and the nature of the monstrous-feminine. The way women are presented and constructed in horror is far different than their male counterparts, as gendered expectations differ wildly. These manuscript illuminations contain combat and potentially horrific situations, but nearly always come off as humorous. I will also be discussing the importance of humor in difficult and potentially problematic situations. Comedy, particularly dark comedy, can scrutinize aspects of culture and society that may otherwise be considered improper taboo to touch upon. Unusual women behaving in an improper manner is a rare subject, and one that deserves to be discussed. By going through historical legends that also combine the usually disparate themes of maternity and violence, I attempt to both explain the image in the Geese Book and frame it in a broader context. The monstrous women from the illumination are compared to both artworks contemporary to the gradual and more modern media. By connecting representations from both the Middle Ages and today, we can better understand where feminine constructions originated and how they have changed. Today, portrayals of the monstrous-feminine have warped traditional maternal archetypes and are unafraid to lay bare the bizarre and grotesque potential of motherhood.

I never feel completely comfortable with someone until I know I can make them laugh. Humor has played an important role in all of my personal relationships, with friends, family and coworkers. For this reason, humor has always fascinated me. One person's sense of humor can differ so greatly from another's, yet the reaction of laughter is the same. Entering college, I saw the field of psychology as the most direct path to studying humor. My thesis was always going to address humor in some way, and I decided that the best way to study humor was through stand-up comedians. These performers spend most of their time trying to make other people laugh, but they don't seem very happy. I decided to watch local shows and interview local comedians, with the goal of better understanding this relationship between humor and sadness. Specifically, I wanted to find out how these comedians use humor to deal with negative experiences in their lives. I conducted interviews with six local stand-up comics, who have experienced varying degrees of success in their stand-up careers. The questions for the interviews were developed to best determine how the comics had decided to work in stand-up comedy, what their career trajectories had looked like, how they develop their material, how humor connects to negative experiences in their lives, and how committed each comic was to performing stand-up. Also, I hoped to gain a better understanding of what role stand-up played in shaping the identity of each comic. Interviews lasted between 40 and 75 minutes. I interviewed the local stand-up comics Iesha Renee, Shapel Lacey, Anwar Newton, Mike Enders and Charles Engle, and Michael Turner.

Soil-transmitted helminthiasis (STH), a neglected tropical disease (NTD) remains a major health problem all over the world especially in developing countries such as, Cameroon with a prevalence of 30.8%, Nigeria and Ghana with a prevalence of 25.4% (Pullan et. al, 2014). This study touches on transmission patterns and investigates the effectiveness of policies on mass drug administration as a means to control STH in Ghana. The government of Ghana currently focuses mass drug administration efforts on school aged children (SAC) that are children between the ages of 5-14 years. This paper develops and evaluates a different mass drug administration strategy by hypothesizing that it would be more cost-effective to target some percentage of vulnerable adults in MDA efforts as opposed to only targeting SAC between ages 5-14 years in Ghana. This we hypothesize would lead to a faster reduction in prevalence over time, would be cost-effective and would hopefully lead to an eventual reduction in morbidity caused by this disease to a level of no public health significance in Ghana. We conduct three cost-effectiveness analyses based on three different case setups. Given the parameter values from literature, our results suggest that it is most cost-effective to cover 20% of adults while covering at least 24% of children in mass drug administration assuming that the number of individuals covered is equal to 80% a figure which is the current total coverage of school-aged children.

The number of undergraduate students participating in short-term experiences in global health (STEGHs) abroad has increased dramatically in recent years (Eyler 2002, Drain et al. 2007). These experiences, in tandem with classroom learning, are designed to help students master skills related to global health competencies, including cultural humility and sensitivity, collaborating with community partners, and sociocultural and political awareness. Although STEGHs offer potential benefits to both students and to sending institutions, these experiences can sometimes be problematic and raise ethical challenges. As the number of students engaged in STEGHs continues to increase, it is important to better understand the impact of these programs on student learning. Current ethical and best practice guidelines for STEGHs state that programs should establish evaluation methods to solicit feedback from students both during and on completion of the program (Crump et al. 2010). However, there is currently no established method for gathering this feedback because of the many different global health competency frameworks, types and duration of programs, and different models of student engagement in such programs. Assessing the quality of a STEGH is a profoundly important and difficult question that cannot be answered as succinctly and quantitatively as classroom performance, which has more standard and established assessment metrics. The goal of this project is to identify the most appropriate and useful assessment metric(s) for determining educational quality and impact for STEGHs at ASU by comparing a typical quantitative evaluation tool (pre-post survey with brief open-ended questions) to a more in-depth qualitative method (key informant interviews). In performing my analysis I seek to examine if the latter can produce a richer narrative of student experiences to inform ongoing program evaluations. My research questions are: 1. What are the current qualitative and quantitative evaluation methods available to assess student learning during short-term experiences in global health? 2. How can current methodology for assessing student experiences with short-term experiences in global health be adapted to collect the most information from students? 3. How do student knowledge and attitudes change before and after their short-term experience in global health? Why is understanding those changes important for adapting programs? My end goal would be to use these new, optimal assessment methods for gathering student perspectives and experiences to adapt pre-departure trainings and post-experience debriefings for study abroad programs, both of which I believe will lead to more sustainable partnerships and a healthier understanding of global health work for students.

Stroke is a devastating disease that affects thousands of individuals each year. Stroke, specifically cerebral ischemia, and immune responses are important areas of study and focus. Previous studies on stroke in mouse models had shown the upregulation of a specific micro-RNA: miR-1224. We hypothesized that miR-1224 was responsible for the regulation of the ST2 receptor protein’s expression. We performed cellular transfection on murine splenocytes with four different miRNAs—miR-1224-mimic, miR-1224-inhibitor, miR-451-mimic, and a control. We predicted that transfection with 1224m would decrease ST2 expression, while transfection with 1224i would increase ST2 expression. Two complete trials were run, and analysis of the results included RT-PCR of both miRNA samples and mRNA samples to confirm transfection and controlled transcription. Reverse transcription and qPCR of miRNA was done in order to confirm that transfection was in fact successful. Reverse transcription and qPCR of the mRNA was done in order to confirm that ST2 mRNA was not altered; this allowed us to attribute any changes in ST2 protein levels to miRNA interactions, as the mRNA levels were consistent. Western blotting was done in order to assess relative protein content. We found that transfection with 1224m slightly decreased ST2 expression and transfection with 1224i slightly increased ST2 expression, however, after assessing the p-values through statistical analyses, neither difference was significant. As such, our hypothesis was rejected as it is not evident that miR-1224 plays a significant role on ST2 gene expression. Future studies are needed in order to analyze alternate protein targets to fully assess the role of miR-1224.

This research aims to look at the lower level collegiate athletics, Intramural sports and club sports, in comparison to Division 1 varsity athletics to see how their sport lives differ and why they are still competing when the reward does not seem as grand as the Varsity athletics. The findings show that the socially ingrained aspect of sports is the reason that most lower level athletes keep competing.

Laboratory animals represent an invaluable, yet controversial, resource in the field of biomedical research. Animal research has been behind many influential discoveries in the field of emerging therapeutics. They provide the link between the theory of the lab bench and the functional application of medicine to influence human health. The use of animals in research is a consideration which must be heavily weighed, and the implementation must be carried out at a very high standard in order to retain research integrity and responsibility. We are in the process of conducting an experiment using laboratory mice to demonstrate cancer treatment using vaccinia (VACV) mutants as a possible oncolytic therapy for certain strains of melanoma. VACV is a double-stranded DNA poxvirus with a large and easily altered genome. This virus contains many genes dedicated to immune evasion, but has shown sensitivity to cell death by necroptosis in mouse studies (5). We have identified the absence of the kinase RIP3 which is vital in the necroptosis pathway as a potential target for oncolytic therapy using VACV mutants in specific strains of melanoma. Multiple groups of SCID Beige mice were inoculated with different melanoma cell lines and observed for tumor growth. Upon reaching 1 cm3 in volume, tumors were injected with either VACV- Δ83N, VACV- Δ54N, or PBS, and observed for regression. It was hypothesized that melanoma tumors that are RIP3-/- such as the MDA5 cell line will show regression, but melanoma tumors that are RIP3-positive and capable of necroptosis, such as the 2427 cell line, will resist viral replication and continue to proliferate. Our results so far tentatively support this hypothesis, but the data collection is ongoing. Strict and specific protocols with regard to the ethical and responsible use of mice have been implemented and upheld throughout the experiment. Animals are closely monitored, and if their quality of life becomes too poor to justify their continued use in the experiment, they are humanely euthanized, even at the expense of valuable data. The importance of commitment to a high ethical standard is pervasive throughout our work. Animals represent an invaluable contribution to research, and it is important to maintain high standards and transparency with regard to their use. Education and engagement in critical discussions about the use and care of animals in the laboratory contribute to the overall merit and legitimacy of biomedical research in the public and professional eye as a whole, and give legitimacy to the continued use of animals as models to advance science and health.