Sustainability challenges with severe local to global impacts require fundamental shifts in what industrial societies aspire to, generate, consume, and represent, as well as how they function. Transition governance is a promising framework to support these transformational efforts. A key component of transition governance is the construction of transition strategies, i.e., action schemes for how to transition from the current state to a sustainable one. Despite accomplishments in building theory and methodology for transition governance, the concepts of what transition strategies entail and how they relate to specific interventions are still underdeveloped. This dissertation further develops the concept of transition strategies, and explores how different stakeholder groups and allies can develop and test transition strategies across different scales, in the specific context of urban sustainability challenges. The overarching research question is: How can cities build and implement comprehensive transition strategies across different urban scales, from the city to the organizational level? The dissertation comprises four studies that explore the dynamic between transition strategies and experiments at the city, neighborhood, and organizational levels with empirical examples from Phoenix, Arizona. The first study reviews and compares paradigms of intentional change, namely transition governance, backcasting, intervention research, change management, integrated planning, and adaptive management in order to offer a rich set of converging ideas on what strategies for intentional change towards sustainability entail. The second study proposes a comprehensive concept of transition strategies and illustrates the concept with the example of sustainability strategies created through a research partnership with the City of Phoenix. The third study explores the role of experiments in transition processes through the lens of the neighborhood-level initiative of The Valley of the Sunflowers. The fourth study examines the role organizations can play in initiating urban sustainability transitions using exemplary strategies and experiments implemented at a local high school. The studies combined contribute to the further development of transition theory and sustainable urban development concepts. While this research field is at a nascent stage, the thesis provides a framework and empirical examples for how to build evidence-based transition strategies in support of urban sustainability.