Endocrine-disrupting chemicals (EDCs) are substances that disrupt the function of the endocrine system by blocking or mimicking hormones. Over the years, these substances have been identified as responsible for producing adverse reproductive effects in freshwater vertebrate populations. Freshwater vertebrates include reptiles, amphibians, and fish living in bodies of water such as lakes or streams and are exposed when concentrations of EDCs enter their habitats. With over 800 known or potential EDCs identified, ample studies can be conducted on the effects of EDCs on freshwater vertebrates; however, studies can be costly. Since studies are costly, I have developed a methodology to prioritize EDC studies. I analyzed ten EDCs to determine their impact on freshwater vertebrates. I specified four criteria and EDCs that passed all four criteria were considered significant. The four criteria I utilized were population decreases, routes of exposure, adverse reproductive effects, and environmental persistence. I analyzed research studies as evidence for the pass or fail of each criterion, where I considered the EDC "ambiguous" if there was not enough information to make a judgment. I then assessed the research available for each EDC. Only one EDC had adequate information to pass or fail each criterion. The one with adequate information passed all criteria. Two EDCs lacked adequate information for three of the four criteria, three EDCs lacked adequate information for two of the four criteria, and four EDCs lacked adequate information for one of the four criteria. I assessed the EDCs based on whether there was adequate information available in each criterion in order to provide researchers direction for future research endeavors. The results indicate either there is much research that remains to be conducted or that researchers are not making existing results of studies available. Companies producing EDCs that are released into the environment can use the information in this report as a basis for determining strategies to minimize the impacts of EDCs on freshwater vertebrates.