This study examined differences in personal definitions of virginity among college students who had received either abstinence-stressing sex education or comprehensive sex education. It was hypothesized that participants who received abstinence-education would be more likely to perceive virginity as a gift, to be unsure about what specific sexual activities result in virginity loss, to have taken formal or personal virginity promises, to define their own non-vaginal sexual activity as abstinent, and to have pretended to be a virgin when they were not. Two surveys were distributed online to students at a large Southwestern university, and responses from 352 quantitative surveys and 75 qualitative surveys were analyzed. Participants in the abstinence group were more likely to be unsure about what sexual activities count as virginity loss and were more likely to have pretended they were a virgin when they were not. Women in the abstinence group were more likely to perceive virginity as a gift than those in the comprehensive group, though men were not. No differences were discerned between the abstinence group and the comprehensive group in rates of formal virginity promises or in tendency to define their own sexual activity as abstinent.