Resistant starch is defined as a portion of starch that bypasses breakdown and absorption in the small intestine. Several studies have shown a relationship between resistant starch consumption and decreased postprandial blood glucose. RS3 in particular, is formed when a starch containing food changes its chemical structure after it is cooked and then cooled. This characteristic enables a food to lower its glycemic index after it has been cooled enough to promote retrogradation and can potentially be used as a dietary intervention to control blood glucose in diabetics and individuals with insulin resistance. Although many studies have been done on rice and potatoes, pasta has not been tested as extensively. This investigation examines the effect of RS3 in pasta on postprandial glycemic response in 13 healthy participants. After consuming 2 bagels the night prior and coming in to the lab in a fasted state, the test subjects were given a warm or cold pasta dish to consume. Blood glucose samples were taken at fasting, 30 minutes, 60 minutes, 90 minutes, and 120 minutes for both the hot and cold dish. Analysis of the results indicated that there was an average of a 4.1 mg/dl decrease in blood glucose at 30 min and a 2.4 mg/dl decrease at 60 min for the cold pasta. This was followed by a 3.9 mg/dl increase at 90 min and 3.3 mg/dl increase at 120 min. Although these results show a small difference in postprandial glucose response, statistical analysis did not deem it significant difference.