The reactive transport related to microbially induced desaturation and precipitation (MIDP) via dissimilatory reduction of nitrogen (denitrification) in a sand layer trapped between the two silt layers was evaluated experimentally. MIDP is an emerging non-disruptive liquefaction mitigation technique that stimulates naturally occurring microorganisms to reduce nitrate to nitrogen gas and oxidize organic carbon to inorganic carbon. The relatively insoluble nitrogen gas desaturates the soil and carbonate ions combine with calcium ions in the pore water and precipitate as calcium carbonate (CaCO3). Both desaturation and carbonate precipitation can mitigate liquefaction potential, but both processes, along with biomass formation, also modify the hydraulic properties of the soil, complicating the treatment process. Several studies have already demonstrated the mechanical response for MIDP treated homogenous granular soils at the bench scale. In addition, tank tests performed by Stallings Young et al. 2021 in coarse sand and stratified sandy soil conditions have been performed to evaluate the reactive transport and treatment performance at meter-scale planar flow conditions in uniform and stratified sand layers. However, there are many instances in the field where liquefiable sand layers are overlain by thin silt layers. Knowledge of the distribution of substrates and products and their effect on the reactive transport in such stratified soil conditions and the longevity of the gas bubbles is limited. In this study, an experiment was performed simulating two-dimensional planar flow conditions to evaluate the condition where a liquefiable sand layer is confined between silt layers. Multiple treatment cycles were employed targeting a maximum iii average CaCO3 content of 1%. Time lapse image analysis of the flow of substrates throughout the process was used to determine seepage velocity and monitor changes in the hydraulic properties of the soil and the migration and persistence of desaturation throughout and after the treatment. The measurement results of various embedded sensors were used to analyze the effectiveness of MIDP treatment and distribution of substrates and products throughout the treated soil with time. Results highlighted various mechanisms by which gas could migrate through the soil.