This thesis explores the human factors effects pilots have when controlling the aircraft during the takeoff phase of flight. These variables come into play in the transitory phase from ground roll to flight, and in the initiation of procedures to abort a takeoff during the ground run. The FAA provides regulations for manufacturers and operators to follow, ensuring safe manufacture of aircraft and pilots that fly without endangering the passengers; however, details regarding accounting of piloting variability are lacking. Creation of a numerical simulation allowed for the controlled variation of isolated piloting procedures in order to evaluate effects on field performance. Reduced rotation rates and delayed reaction times were found to cause significant increases in field length requirements over values published in the AFM. A pilot survey was conducted to evaluate common practices for line pilots in the field, which revealed minimum regulatory compliance is exercised with little to no feedback on runway length requirements. Finally, observation of pilots training in a CRJ-200 FTD gathered extensive information on typical piloting timings in the cockpit. AEO and OEI takeoffs were observed, as well as RTOs. Pilots showed large variability in procedures and timings resulting in significant inconsistency in runway distances used as well as V-speed compliance. The observed effects from pilot timing latency correlated with the numerical simulation increased field length outputs. Variability in piloting procedures results in erratic field performance that deviates from AFM published values that invite disaster in an aircraft operating near its field performance limitations.