With high potential for automobiles to cause air pollution and greenhouse gas emissions, there is concern that automobiles accessing or egressing public transportation may cause emissions similar to regular automobile use. Due to limited literature and research that evaluates and discusses environmental impacts from first and last mile portions of transit trips, there is a lack of understanding on this topic. This research aims to comprehensively evaluate the life cycle impacts of first and last mile trips on multimodal transit. A case study of transit and automobile travel in the greater Los Angeles region is evaluated by using a comprehensive life cycle assessment combined with regional household travel survey data to evaluate first-last mile trip impacts in multimodal transit focusing on automobile trips accessing or egressing transit. First and last mile automobile trips were found to increase total multimodal transit trip emissions by 2 to 12 times (most extreme cases were carbon monoxide and volatile organic compounds). High amounts of coal-fired energy generation can cause electric propelled rail trips with automobile access or egress to have similar or more emissions (commonly greenhouse gases, sulfur dioxide, and mono-nitrogen oxides) than competing automobile trips, however, most criteria air pollutants occur remotely. Methods to reduce first-last mile impacts depend on the characteristics of the transit systems and may include promoting first-last mile carpooling, adjusting station parking pricing and availability, and increased emphasis on walking and biking paths in areas with low access-egress trip distances.