Pathogen reduction is a viable approach to ensure the continued safety of the blood supply against emerging pathogens. However, the currently licensed pathogen reduction techniques are ineffective against non-enveloped viruses such as hepatitis A virus, and they introduce chemicals with concerns of side effects which prevent their widespread use. In this report, we demonstrate the inactivation of both enveloped and non-enveloped viruses in human plasma using a novel chemical-free method, a visible ultrashort pulsed laser. We found that laser treatment resulted in 2-log, 1-log, and 3-log reductions in human immunodeficiency virus, hepatitis A virus, and murine cytomegalovirus in human plasma, respectively. Laser-treated plasma showed ≥70% retention for most coagulation factors tested. Furthermore, laser treatment did not alter the structure of a model coagulation factor, fibrinogen. Ultrashort pulsed lasers are a promising new method for chemical-free, broad-spectrum pathogen reduction in human plasma.
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- Pathogen Reduction in Human Plasma Using an Ultrashort Pulsed Laser
- Tsen, Shaw-Wei D. (Author)
- Kingsley, David H. (Author)
- Kibler, Karen (Author)
- Jacobs, Bertram (Author)
- Sizemore, Sara (Author)
- Vaiana, Sara (Author)
- Anderson, Jeanne (Author)
- Tsen, Kong-Thon (Author)
- Achilefu, Samuel (Author)
- Biodesign Institute (Contributor)
- Digital object identifier: 10.1371/journal.pone.0111673
- Identifier TypeInternational standard serial numberIdentifier Value1045-3830
- Identifier TypeInternational standard serial numberIdentifier Value1939-1560
- The article is published at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0111673
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Tsen S-WD, Kingsley DH, Kibler K, Jacobs B, Sizemore S, et al. (2014) Pathogen Reduction in Human Plasma Using an Ultrashort Pulsed Laser. PLoS ONE 9(11): e111673. doi:10.1371/journal.pone.0111673