Background: Hepatitis C virus (HCV) coinfection was reported to negatively affect HIV disease and HIV infection has a deleterious effect on HCV-related liver disease. However, despite common occurrence of HCV/HIV coinfection little is known about the mechanisms of interactions between the two viruses.
Methods: We studied CD4+ and CD8+ T cell and CD19+ B cell apoptosis in 104 HIV-positive patients (56 were also HCV-positive) and in 22 HCV/HIV-coinfected patients treated for chronic hepatitis C with pegylated interferon and ribavirin. We also analyzed HCV/HIV coinfection in a Daudi B-cell line expressing CD4 and susceptible to both HCV and HIV infection. Apoptosis was measured by AnnexinV staining.
Results: HCV/HIV coinfected patients had lower CD4+ and CD8+ T cell apoptosis and higher CD19+ B cell apoptosis than those with HIV monoinfection. Furthermore, anti-HCV treatment of HCV/HIV coinfected patients was followed by an increase of CD4+ and CD8+ T cell apoptosis and a decrease of CD19+ B cell apoptosis. In the Daudi CD4+ cell line, presence of HCV infection facilitated HIV replication, however, decreased the rate of HIV-related cell death.
Conclusion: In HCV/HIV coinfected patients T-cells were found to be destroyed at a slower rate than in HIV monoinfected patients. These results suggest that HCV is a molecular-level determinant in HIV disease.
Details
- Effect of Hepatitis C Infection on HIV-Induced Apoptosis
- Laskus, Tomasz (Author)
- Kibler, Karen (Author)
- Chmielewski, Marcin (Author)
- Wilkinson, Jeffrey (Author)
- Adair, Debra (Author)
- Horban, Andrzej (Author)
- Stanczak, Grzegorz (Author)
- Radkowski, Marek (Author)
- ASU Biodesign Center Immunotherapy, Vaccines and Virotherapy (Contributor)
- Biodesign Institute (Contributor)
- Digital object identifier: 10.1371/journal.pone.0075921
- Identifier TypeInternational standard serial numberIdentifier Value1045-3830
- Identifier TypeInternational standard serial numberIdentifier Value1939-1560
- The article is published at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0075921
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Laskus, T., Kibler, K. V., Chmielewski, M., Wilkinson, J., Adair, D., Horban, A., . . . Radkowski, M. (2013). Effect of Hepatitis C Infection on HIV-Induced Apoptosis. PLoS ONE, 8(10). doi:10.1371/journal.pone.0075921