Description

There is an increasing awareness that health care must move from post-symptomatic treatment to presymptomatic intervention. An ideal system would allow regular inexpensive monitoring of health status using circulating antibodies to report on health fluctuations. Recently, we demonstrated that peptide

There is an increasing awareness that health care must move from post-symptomatic treatment to presymptomatic intervention. An ideal system would allow regular inexpensive monitoring of health status using circulating antibodies to report on health fluctuations. Recently, we demonstrated that peptide microarrays can do this through antibody signatures (immunosignatures). Unfortunately, printed microarrays are not scalable. Here we demonstrate a platform based on fabricating microarrays (~10 M peptides per slide, 330,000 peptides per assay) on silicon wafers using equipment common to semiconductor manufacturing. The potential of these microarrays for comprehensive health monitoring is verified through the simultaneous detection and classification of six different infectious diseases and six different cancers. Besides diagnostics, these high-density peptide chips have numerous other applications both in health care and elsewhere.

Reuse Permissions
  • Downloads
    PDF (906.9 KB)

    Details

    Title
    • Scalable High-Density Peptide Arrays for Comprehensive Health Monitoring
    Contributors
    Date Created
    2014-09-03
    Resource Type
  • Text
  • Collections this item is in
    Identifier
    • Digital object identifier: 10.1038/ncomms5785
    • Identifier Type
      International standard serial number
      Identifier Value
      2041-1723
    Note
    • The final version of this article, as published in Nature Communications, can be viewed online at: http://www.nature.com/articles/ncomms5785

    Citation and reuse

    Cite this item

    This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.

    Legutki, J. B., Zhao, Z., Greving, M., Woodbury, N., Johnston, S. A., & Stafford, P. (2014). Scalable high-density peptide arrays for comprehensive health monitoring. Nature Communications, 5, 4785. doi:10.1038/ncomms5785

    Machine-readable links