The Philippines relies on a vast biodiversity of fishes as a staple food, but like many countries around the globe, experiences severe “leakages” of contaminants and pollutants in the environment. In order to better understand the relationship between environmental pollutants and public health, this research project measured the concentration of pollutants in a commonly consumed local fish (Siganus fuscescens), and then evaluated the potential health risks of eating this fish based on estimated average consumer weight and consumption levels. Fish sampled from four different sites located in Negros Oriental, Philippines were analyzed for organic contaminants using gas chromatography and mass spectroscopy. Pollutants quantified included polycyclic aromatic hydrocarbons (PAHs), pesticides, phthalates, and polychlorinated biphenyl (PCBs). Across the four study sites, fishes from Manjuyod showed the highest frequency of detection of different pollutants. However, phthalates and PAHs were found in similar concentrations in all four sites, with fishes from Dumaguete showing the highest level of PCBs compared to the other sampled sites. The U.S. Environmental Protection Agency’s guide for fish contaminants pinpoints several health risks associated with the chronic ingestion of these contaminants. Based on estimated average body weights of Filipino adult men, adult women, and children, and various consumption levels, people who eat the fish at or above the national average consumption level may be at increased risk for chronic health outcomes, such as cancer and/or other adverse effects. Specifically, due to the high concentration of PCBs in Dumaguete, selected populations who eat local fish from this site may be at higher risk than the citizens who eat the fish from other sites at similar consumption rates. These results can help to inform local and national policies on water quality, waste disposal, and fish consumption advisory programs.

This thesis utilizes GIS mapping to analyze the severity of four threats: ocean acidification, sea surface temperature, artisanal fishing, and destructive fishing, in conjunction with coral species distribution. This project produced maps that depicts each of these threats and shows the distribution of its severity. Compiling this data we can see that ocean acidification is the most pressing threat in the Caribbean to coral and that neither type of fishing really has a large effect. A species named Madracis carmabi is also flagged to be of particular concern as it is severely threatened by both ocean acidification and sea surface temperature.

Plastic pollution has become a global threat to ecosystems worldwide, with microplastics now representing contaminants reported to occur in ambient air, fresh water, seawater, soils, fauna and people. Over time, larger macro-plastics are subject to weathering and fragmentation, resulting in smaller particles, termed ‘microplastics’ (measuring < 5 mm in diameter), which have been found to pollute virtually every marine and terrestrial ecosystem on the planet. This thesis explored the transfer of plastic pollutants from consumer products into the built water environment and ultimately into global aquatic and terrestrial ecosystems.

A literature review demonstrated that municipal sewage sludge produced by wastewater treatment plants around the world contains detectable quantities of microplastics. Application of sewage sludge on land was shown to represent a mechanism for transfer of microplastics from wastewater into terrestrial environments, with some countries reporting as high as 113 ± 57 microplastic particles per gram of dry sludge.

To address the notable shortcoming of inconsistent reporting practices for microplastic pollution, this thesis introduced a novel, online calculator that converts the number of plastic particles into the unambiguous metric of mass, thereby making global studies on microplastic pollution directly comparable.

This thesis concludes with an investigation of a previously unexplored and more personal source of plastic pollution, namely the disposal of single-use contact lenses and an assessment of the magnitude of this emerging source of environmental pollution. Using an online survey aimed at quantifying trends with the disposal of lenses in the US, it was discovered that 20 ± 0.8% of contact lens wearers flushed their used lenses down the drain, amounting to 44,000 ± 1,700 kg y-1 of lens dry mass discharged into US wastewater.

From the results it is concluded that conventional and medical microplastics represent a significant global source of pollution and a long-term threat to ecosystems around the world. Recommendations are provided on how to limit the entry of medical microplastics into the built water environment to limit damage to ecosystems worldwide.

Fish consumption advisories are important to the protection of human health but are not often widely communicated nor systematically or comprehensively conducted. The objective of this thesis is to explore the strengths and weaknesses of current fish consumption advisory programs in the US and Canada, providing a comparison between the two countries. The US and Canada were chosen because the two countries are relatively similar in socio-economic makeup as well as in their state vs. federal regulatory setup, allowing for easier comparison. At the sub-federal level, Arizona was chosen to serve as a case study for the US, and Nova Scotia as a case study for Canada. To compare each country, fish consumption advisory programs were broadly described across the 50 US states and 13 Canadian provinces and territories to provide a full understanding of the variation in such programs within each country. In addition to comparison across states and provinces, opportunities and challenges for policy correction to strengthen fish consumption advisory programs will be provided, including suggestions on how the US and Canada can learn from each other in creating better environmental policy. Policy is addressed as a means of improving fish consumption advisory programs because without state or federal requirements to monitor fish tissue for contaminants of concern across the US and Canada, there is no guarantee human health and environmental justice will remain protected in either country. Potential global sources of enhanced environmental policy will be provided as examples of further opportunities for the US and Canada to improve fish consumption advisory program policies.

In an industrialized world that relies heavily on low cost production and packaging produced without a viable end-of-life strategy, the accumulation of non-biodegradable plastics in the environment and particularly the oceans today is an urgent problem of global proportions. Plastics pose a significant threat to marine mammals due to mistaken ingestion as well as potential release of plasticizers and other chemicals. However, the interactions and consequences of ingestion of oceanic plastics by marine mammals have not been thoroughly studied. In this literature review, information regarding plastic ingestion by marine mammals was compiled to estimate the magnitude of adverse impacts and identify major knowledge gaps. Using comprehensive Boolean search terms in Web of Science of literature published between 1960 and 2020, it was determined that there were large discrepancies in the amount of research conducted among 10 different categories of marine mammals, with cetaceans being the primary focus group of most studies (70.3). In addition, different areas of the world, such as southern Africa, were found to have a disproportionately small number of studies on plastic impacts on marine mammalian life in their surrounding marine waters. Differences were found in the amount of plastics ingested by marine animals and a hypothesis of explaining these observations was formulated, attributing potential ingestion of plastic to the debris resembling different food sources in the mammals’ diets as well as different feeding mechanisms.

The Gulf of Mexico (or “Gulf”) is of critical significance to the oil and gas industries’ offshore production, but the potential for accidental petrochemical influx into the Gulf due to such processes is high; two of the largest marine oil spills in history, Pemex's Ixtoc I spill (1979) and British Petroleum's (BP) Deepwater Horizon (2010), have occurred in the region. However, the Gulf is also of critical significance to thousands of unique species, many of which may be irreparably harmed by accidental petrochemical exposure. To better manage the conservation and recovery of marine species in the Gulf ecosystem, a Petrochemical Vulnerability Index was developed to determine the potential impact of a petrochemical influx on Gulf marine fishes, therein providing an objective framework with which to determine the best immediate and long term management strategies for resource managers and decision-makers. The resulting Petrochemical Vulnerability Index (PVI) was developed and applied to all bony fishes and shark/ray species in the Gulf of Mexico (1,670 spp), based on a theoretical petrochemical vulnerability framework developed by peer review. The PVI for fishes embodies three key facets of species vulnerability: likelihood of exposure, individual sensitivity, and population resilience, and comprised of 11 total metrics (Distribution, Longevity, Mobility, Habitat, Pre-Adult Stage Length, Pre-Adult Exposure; Increased Adult Sensitivity Due to UV Light, Increased Pre-Adult Sensitivity Due to UV Light; and Abundance, Reproductive Turnover Rate, Diet/Habitat Specialization). The resulting PVI can be used to guide attention to the species potentially most in need of immediate attention in the event of an oil spill or other petrochemical influx, as well as those species that may require intensive long-term recovery. The scored relative vulnerability rankings can also provide information on species that ought to be the focus of future toxicological research, by indicating which species lack toxicological data, and may potentially experience significant impacts.

Cetacean-based ecotourism is a popular activity and an important source of revenue for many countries. Whale watching, a subset of cetacean-based ecotourism, is vital to supporting conservation efforts and provides numerous benefits to local communities including educational opportunities and job creation. However, the sustainability of whale-based ecotourism depends on the behavior and health of whale populations and is therefore vital that ecotourism industries consider the impact their activities have on whale reproductive behavior. To address this statement, behavioral data (e.g. direction change, breaching, slap behaviors, diving, and spy hops) were collected from humpback whales (Megaptera novaeangliae) in Las Perlas Archipelago off the Pacific coast of Panama to determine if vessel presence had an influence on whale behaviors. Studies were recorded during their breeding season from August through September in 2019. Based on 47 behavioral observations, higher boat density corresponded with humpback whales changing direction which is believed to be a sign of disturbance. This result is important given Panamanian regulations implemented on February 13 of 2007 prohibit whale-based tourism from disturbing whales, which is measured as changes in behavior. Because there is no systematic monitoring of whale watching activity to enforce the regulations, there is currently little compliance among tour operators. The integration of animal behavior research into management planning will result in more effective regulation and compliance of conservation policies.

The prevalence of plastic products has exponentially grown over the past two decades. Single-use plastics are the largest source of plastic waste in the environment and are heavily produced for both food and drink packaging. While it is argued that single-use plastics maintain product longevity and are associated with ‘ease of use’, this research paper questions their essentiality.

In this study, single-use plastics in the food packaging industry were systematically reviewed in order to determine their ‘essentiality’ for product longevity. Four grocery stores were chosen and their brands ‘in plastic’ and ‘not in plastic’ were counted. Seven subcategories of food and drink types were created such that a proportion was representative of the brands in plastic per category.

The results of the systematic review showed that the majority of categories sampled from in each store had at least 80% of their brands in plastic packaging. Across four of the seven subcategories, 99-100% of each stores’ brands were in plastic packaging. Furthermore, six alternatives to single-use plastic packaging were reviewed and compared to current methods of food and drink packaging. This comparison deemed that none of the single-use packaging methods utilized in grocery stores were considered essential.

While this study concluded that none of the single-use plastics reviewed were deemed essential, alternatives still remain at a higher cost of production. Further innovation and widespread production of safe alternatives are both integral factors in reducing plastic production and protecting the future of the environment.

Biodiversity is required to guarantee proper ecosystem structure and function. However, increasing anthropogenic threats are causing biodiversity loss around the world at an unprecedented rate, in what has been deemed the sixth mass extinction. To counteract this crisis, conservationists seek to improve the methods used in the design and implementation of protected areas, which help mitigate the impacts of human activities on species. Marine mammals are ecosystem engineers and important indicator species of ocean and human wellbeing. They are also disproportionally less known and more threatened than terrestrial mammals. Therefore, surrogates of biodiversity must be used to maximize their representation in conservation planning. Some of the most effective surrogates of biodiversity known have only been tested in terrestrial systems. Here I test complementarity, rarity, and environmental diversity as potential surrogates of marine mammal representation at the global scale, and compare their performance against species richness, which is the most popular surrogate used to date. I also present the first map of marine mammal complementarity, and assess its relationship with environmental variables to determine if environmental factors could also be used as surrogates. Lastly, I determine the global complementarity-based hotspots of marine mammal biodiversity, and compare their distributions against current marine protected area coverage and exposure to global indices of human threats, to elucidate the effectiveness of current conservation efforts. Results show that complementarity, rarity, and environmental diversity are all efficient surrogates, as they outcompete species richness in maximizing marine mammal species representation when solving the minimum-set coverage problem. Results also show that sea surface temperature, density, and bathymetry are the top environmental variables most associated with complementarity of marine mammals. Finally, gap analyses show that marine mammals are overall poorly protected, yet moderately exposed to hotspots of cumulative human impacts. The wide distribution of marine mammals justify global studies like the ones here presented, to determine the best strategy for their protection. Overall, my findings show that less popular surrogates of biodiversity are more effective for marine mammals and should be considered in their management, and that the expansion of protected areas in their most important habitats should be prioritized.

Marine conservation faces the unique challenge of trying to assess and protect species, like sharks, that have long migration tracks and are often targeted by fishing vessels in open and international waters. Over the last two decades, several large predatory shark populations have been greatly depleted despite local and international organizations designed to help regulate and prevent predator removal to avoid disturbing the food web those sharks balance (Myers, Baum, Shepherd, Powers, & Peterson, 2007). Forensic science is a powerful tool that could give shark conservation efforts an edge on identifying shark species currently being targeted by unsustainable fisheries in international waters. Allowing offenders who break international conservation laws to be prosecuted for their crimes. Unfortunately, this unique and powerful tool has not been given the opportunity to be utilized as it should be. An overview of national and international agencies, organizations, and laws disclosed a strong foundation for wildlife conservation. However, current international organizations and laws that govern international waters leave much to be desired in regards to protecting shark species that are threatened due to being popular targets for fishing vessels. This paper examines the level of forensic science involvement in shark conservation efforts through a literature review, revealing a severe lack of real-life application of forensic science to marine conservation cases. Current issues that marine wildlife forensic science encounters while attempting to increase forensic capability. And finally, presenting proposals for the future, and new challenges, which aim to strengthen the relationship between forensic science and marine conservation.