The potential of using bio-geo-chemical processes for applications in geotechnical engineering has been widely explored in order to overcome the limitation of traditional ground improvement techniques. Biomineralization via urea hydrolysis, referred to as Microbial or Enzymatic Induced Carbonate Precipitation (MICP/EICP), has been shown to increase soil strength by stimulating precipitation of calcium carbonate minerals, bonding soil particles and filling the pores. Microbial Induced Desaturation and Precipitation (MIDP) via denitrification has also been studied for its potential to stabilize soils through mineral precipitation, but also through production of biogas, which can mitigate earthquake induced liquefaction by desaturation of the soil. Empirical relationships have been established, which relate the amount of products of these biochemical processes to the engineering properties of treated soils. However, these engineering properties may vary significantly depending on the biomineral and biogas formation mechanism and distribution patterns at pore-scale. This research focused on the pore-scale characterization of biomineral and biogas formations in porous media.

The pore-scale characteristics of calcium carbonate precipitation via EICP and biogenic gas formation via MIDP were explored by visual observation in a transparent porous media using a microfluidic chip. For this purpose, an imaging system was designed and image processing algorithms were developed to analyze the experimental images and detect the nucleation and growth of precipitated minerals and formation and migration mechanisms of gas bubbles within the microfluidic chip. Statistical analysis was performed based on the processed images to assess the evolution of biomineral size distribution, the number of precipitated minerals and the porosity reduction in time. The resulting images from the biomineralization study were used in a numerical simulation to investigate the relation between the mineral distribution, porosity-permeability relationships and process efficiency. By comparing biogenic gas production with abiotic gas production experiments, it was found that the gas formation significantly affects the gas distribution and resulting degree of saturation. The experimental results and image analysis provide insight in the kinetics of the precipitation and gas formation processes and their resulting distribution and related engineering properties.

The experience base of practitioners with expansive soils is largely devoid of directly measured soil suction. This historical lack of soil suction measurement represents an impediment to adoption of modern unsaturated soil engineering to problems of expansive soils. Most notably, soil suction-based analyses are paramount to proper design of foundations in expansive soils. Naturally, the best method to obtain design suction profiles is to perform an appropriate geotechnical investigation that involves soil moisture change-appropriate drilling depths, sampling intervals, and requisite laboratory testing, including suction measurement. However, as practitioners are slow to embrace changes in methodology, specifically regarding the adoption of even relatively simple suction measurement techniques, it has become imperative to develop a method by which the routine geotechnical procedures currently employed can be used to arrive at acceptable approximations of soil suction profiles.

Herein, a substitute, or surrogate, for soil suction is presented, such that the surrogate agrees with observed field soil suction patterns and provides estimates of soil suction that are acceptable for use in practice. Field investigations with extensive laboratory testing, including direct suction measurement, are used in development of the soil suction surrogate. This surrogate, a function of water content and routinely measured soil index properties, is then used in estimation of field expansive soil suction values. The suction surrogate, together with existing geotechnical engineering reports, is used to augment the limited existing database of field soil suction profiles. This augmented soil suction profile database is used in development of recommendations for design suction envelopes and design suction profiles. Using the suction surrogate, it is possible to proceed from the beginning to the end of the Suction-Oedometer soil heave/shrinkage analysis without directly measuring soil suction. The magnitude of suction surrogate-based heave estimates is essentially the same as heave estimates obtained using direct soil suction measurements.

The soil suction surrogate-based approach, which uses a complete-stress-state approach, considering both net normal stress and soil suction, is an intermediate step towards the adoption of unsaturated soil engineering in expansive soils analyses, wherein direct soil suction measurements are routinely made.

The public has expressed a growing desire for more sustainable and green technologies to be implemented in society. Bio-cementation is a method of soil improvement that satisfies this demand for sustainable and green technology. Bio-cementation can be performed by using microbes or free enzymes which precipitate carbonate within the treated soil. These methods are referred to as microbial induced carbonate precipitation (MICP) and enzyme induced carbonate precipitation (EICP). The precipitation of carbonate is the formation of crystalline minerals that fill the void spaces within a body of soil.

This thesis investigates the application of EICP in a soil collected from the Arizona State University Polytechnic campus. The surficial soil in the region is known to be a clayey sand. Both EICP and MICP have their limitations in soils consisting of a significant percentage of fines. Fine-grained soils have a greater surface area which requires the precipitation of a greater amount of carbonate to increase the soil’s strength. EICP was chosen due to not requiring any living organisms during the application, having a faster reaction rate and size constraints.

To determine the effectiveness of EICP as a method of improving a soil with a significant amount of fines, multiple comparisons were made: 1) The soil’s strength was analyzed on its own, untreated; 2) The soil was treated with EICP to determine if bio-cementation can strengthen the soil; 3) The soil had sand added to reduce the fines content and was treated with EICP to determine how the fines percentage effects the strength of a soil when treated with EICP.

While the EICP treatment increased the strength of the soil by over 3-fold, the strength was still relatively low when compared to results of other case studies treating sandy soils. More research could be done with triaxial testing due to the samples of the Polytechnic soil’s strength coming from capillarity.

Expansive clay soils, when subjected to substantial moisture change, can be extremely problematic causing various types of damage to lightly-loaded structures. Solving these problems requires an understanding of unsaturated soil mechanics. Soil suction, related to moisture content change, is important in the development of unsaturated soil properties and in the assessment of initial and final stress for heave computation. Direct measurement of soil suction on expansive clays to determine field suction profiles is quite limited due primarily to tradition and cost-driven geotechnical field investigation practices prioritizing water content measurement over soil suction measurement. This study employs a surrogate to estimate soil suction profiles for various sites consisting of clay soils with a Plasticity Index of greater than 15. The soil suction surrogate was used to determine suction profiles from existing geotechnical engineering expansive clay field investigations and a limited amount of directly measured suction profiles were also used. Equilibrium suction magnitudes and the depths to constant suction were obtained from the field suction profiles and results were compared to data found in the existing literature. Thornthwaite Moisture Index (TMI) is a climatic index to describe climatic conditions for a given region. Surface flux boundary conditions (i.e. covered and uncovered and irrigated and non-irrigated) were investigated and comparisons were made to the extent possible. Previous studies have presented correlations between TMI and equilibrium suction and TMI and depth to constant suction. Relationships within this study are presented and comparisons are made to existing relationships. Results and recommendations for further research are discussed.

A large portion of the United States is known to have problematic expansive clay soil. These expansive clay soils can cause damage to major infrastructures such as roads and lightly loaded residential buildings. The shrinking or swelling potential of unsaturated expansive clay soils requires an understanding of unsaturated soil mechanics, such as matric suction profile and the site’s environmental condition, such as climate. In unsaturated soil engineering, the most used climatic parameter is Thornthwaite Moisture Index (TMI). Since its inception, there have been several versions of TMI models in the literature. Historically, TMI is used to predict suction parameters such as edge moisture variation length, the depth to equilibrium suction, and equilibrium suction. Currently, TMI is used in Post-Tension Institute’s Slab-on-grade Design Manual (DC 10.1-08) to estimate edge moisture variation length and equilibrium suction, and Australian Standard Residential Slabs and Footing (AS2870-2011) to predict the climatic zone and the depth to suction change. However, there is no clear-cut guidance on which version of TMI models to use, how the variables within TMI should be collected, the length of the study period for determination of TMI, or assumptions and compromises associated with TMI estimation methods. In this thesis, broad-scale study and comparison of the original TMI (1948) to the newer TMI models for the contiguous United States are conducted as well as in-depth analysis of the variables within TMI, using National Oceanic and Atmospheric Administration’s (NOAA) dataset and Geographic Information System (GIS). The results of the study, the recommendations for the state of practice for TMI and further research are discussed.

Expansive soils in the United States cause extensive damage to roadways, buildings, and various structures. There are several treatment or methods of mitigation for these expansive soils. These treatments can be physical or chemical treatments that serve to provide more suitable building qualities for foundations and roadways alike. The main issue with expansive soils, is the volumetric variations, which are known as swelling and consolidation. These behaviors of the soil are usually stabilized through the use of lime solution, Portland Cement Concrete, and a newer technology in chemical treatments, sodium silicate solutions. Although the various chemical treatments show benefits in certain areas, the most beneficial method for stabilization comes from the combination of the chemical treatments. Lime and Portland cement concrete are the most effective in terms of increasing compressive strength and reduction of swell potential. However, with the introduction of silicate into either treatment, the efficacy of the treatments increases by a large amount lending itself more as an additive for the former processes. Sodium silicate solution does not lend itself to effectively increase the compressive strength of expansive soils. The sodium silicate solution treatment needs extensive research and development to further improve the process. A proposed experiment plan has been recommended to develop trends of pH and temperature and its influence on the effectiveness of the treatment. Nonetheless, due to the high energy consumption of the other processes, sodium silicate solution may be a proper step in decreases the carbon footprint, that is currently being created by the synthesis of Portland Cement Concrete and lime.

Laterally-loaded short rigid drilled shaft foundations are the primary foundation used within the electric power transmission line industry. Performance of these laterally loaded foundations is dependent on modulus of the subsurface, which is directly measured by the Pressuremeter (PMT). The PMT test provides the lateral shear modulus at intermediate strains, an equivalent elastic modulus for lateral loading, which mimics the reaction of transmission line foundations within the elastic range of motion. The PMT test, however, is expensive to conduct and rarely performed. Correlations of PMT to blow counts and other index properties have been developed but these correlations have high variability and may result in unconservative foundation design. Variability in correlations is due, in part, because difference of the direction of the applied load and strain level between the correlated properties and the PMT. The geophysical shear wave velocity (S-wave velocity) as measured through refraction microtremor (ReMi) methods can be used as a measure of the small strain, shear modulus in the lateral direction. In theory, the intermediate strain modulus of the PMT is proportional to the small strain modulus of S-wave velocity. A correlation between intermediate strain and low strain moduli is developed here, based on geophysical surveys conducted at fourteen previous PMT testing locations throughout the Sonoran Desert of central Arizona. Additionally, seasonal variability in S-wave velocity of unsaturated soils is explored and impacts are identified for the use of the PMT correlation in transmission line foundation design.

Design and mitigation of infrastructure on expansive soils requires an understanding of unsaturated soil mechanics and consideration of two stress variables (net normal stress and matric suction). Although numerous breakthroughs have allowed geotechnical engineers to study expansive soil response to varying suction-based stress scenarios (i.e. partial wetting), such studies are not practical on typical projects due to the difficulties and duration needed for equilibration associated with the necessary laboratory testing. The current practice encompasses saturated “conventional” soil mechanics testing, with the implementation of numerous empirical correlations and approximations to obtain an estimate of true field response. However, it has been observed that full wetting rarely occurs in the field, leading to an over-conservatism within a given design when partial wetting conditions are ignored. Many researchers have sought to improve ways of estimation of soil heave/shrinkage through intense studies of the suction-based response of reconstituted clay soils. However, the natural behavior of an undisturbed clay soil sample tends to differ significantly from a remolded sample of the same material.

In this study, laboratory techniques for the determination of soil suction were evaluated, a methodology for determination of the in-situ matric suction of a soil specimen was explored, and the mechanical response to changes in matric suction of natural clay specimens were measured. Suction-controlled laboratory oedometer devices were used to impose partial wetting conditions, similar to those experienced in a natural setting. The undisturbed natural soils tested in the study were obtained from Denver, CO and San Antonio, TX.

Key differences between the soil water characteristic curves of the undisturbed specimen test compared to the conventional reconstituted specimen test are highlighted. The Perko et al. (2000) and the PTI (2008) methods for estimating the relationship between volume and changes in matric suction (i.e. suction compression index) were evaluated by comparison to the directly measured values. Lastly, the directly measured partial wetting swell strain was compared to the fully saturated, one-dimensional, oedometer test (ASTM D4546) and the Surrogate Path Method (Singhal, 2010) to evaluate the estimation of partial wetting heave.

After describing the types of foundation systems employed for high rise buildings, this thesis discusses the process of foundation design for tall buildings as it is practiced today, including computer programs used in designing the foundations of high rise buildings. This thesis then presents the geotechnical in-situ and laboratory tests used to establish the parameters required for input to design analyses for high rise building foundations. This thesis subsequently describes the Construction Quality Assurance practices used in the construction of the foundations of high rise buildings. This thesis next presents several case histories detailing the foundation practices employed in the design and construction of modern high rise buildings. Finally, this thesis provides some concluding thoughts regarding the development of the geotechnical practices when designing and constructing high rise buildings.

This dissertation presents an investigation of calcium carbonate precipitation via hydrolysis of urea (ureolysis) catalyzed by plant-extracted urease enzyme for soil improvement. In this approach to soil improvement, referred to as enzyme induced carbonate precipitation (EICP), carbonate minerals are precipitated within the soil pores, cementing soil particles together and increasing the dilatancy of the soil. EICP is a bio-inspired solution to improving the properties of cohesionless soil in that no living organisms are engaged in the process, though it uses a biologically-derived material (urease enzyme).

Over the past decade, research has commenced on biologically-mediated solutions like microbially induced carbonate precipitation (MICP) and biologically-inspired solutions like EICP for non-disruptive ground improvement. Both of these approaches rely upon hydrolysis of urea catalyzed by the enzyme urease. Under the right environmental conditions (e.g., pH), the hydrolysis of urea leads to calcium carbonate precipitation in the presence of Ca^(2+). The rate of carbonate precipitation via hydrolysis of urea can be up to 〖10〗^14 times faster than natural process.

The objective of this research was to ascertain the effectiveness of EICP for soil improvement via hydrolysis of urea (ureolysis) catalyzed by plant-extracted urease enzyme. Elements of this work include: 1) systematic experiments to identify an optimum EICP treatment solution; 2) evaluation of the mechanical properties of EICP-treated soil under different treatment conditions and with varying carbonate contents; 3) investigation of the potential for enhancing the EICP stabilization process by including xanthan gum, natural sisal fiber, and powdered of dried non-fat milk in the EICP treatment solution; and 4) bench-scale studies of the use of EICP to make sub-horizontal columns of cemented soil for soil nailing and vertical columns of cemented soil for foundation support. As part of this research, the effect of three preparation methods (mix-and-compact, percolation, and injection) was also examined as was the influence of the grain size of soil. The results of this study should help make the EICP technique an attractive option for geotechnical engineers for ground improvement and stimulate the development and use of other biogeotechnical techniques for civil engineering purposes.