Cognitive functioning is an extremely crucial part of daily living. In individuals with Down Syndrome (DS) these tasks get even more challenging. The aim of this study is to determine the effects of Assisted Cycling Therapy (ACT) on cognitive functions in children with Down Syndrome (DS). This study examines the change in cognitive functioning using tests like Reaction time, Tower of London, and Card Sorting over an eight week intervention. All seven participants in the study were assigned to complete the ACT intervention, in which they rode a stationary bike with the assistance of a motor to maintain a cadence of at least 35% greater than their voluntary cycling speed. All participants completed the ACT intervention but a few were unable to complete some cognitive functioning tests due to their intellectual abilities. Overall, the results of this study showed that information processing, task-switching and problem solving improved following the eight week ACT intervention. These results provided more scope for future research in this field which can be done by modifying the time period of the intervention, increasing sample size of the study as well as conducting additional cognitive function tests. The results of our study are discussed with respect to the upward regulation of neurotrophic factors which are involved in increasing the functioning within the prefrontal cortex following exercise intervention.