Matching Items (43,913)
Description
A new challenge on the horizon is to utilize the large amounts of protein found in the atmosphere to identify different organisms from which the protein originated. Included here is work investigating the presence of identifiable patterns of different proteins collected from the air and biological samples for the purposes of remote identification. Protein patterns were generated using high performance liquid chromatography (HPLC). Patterns created could identify high-traffic and low-traffic indoor spaces. Samples were collected from the air using air pumps to draw air through a filter paper trapping particulates, including large amounts of shed protein matter. In complimentary research aerosolized biological samples were collected from various ecosystems throughout Ecuador to explore the relationship between environmental setting and aerosolized protein concentrations. In order to further enhance protein separation and produce more detailed patterns for the identification of individual organisms of interest; a novel separation device was constructed and characterized. The separation device incorporates a longitudinal gradient as well as insulating dielectrophoretic features within a single channel. This design allows for the production of stronger local field gradients along a global gradient allowing particles to enter, initially transported through the channel by electrophoresis and electroosmosis, and to be isolated according to their characteristic physical properties, including charge, polarizability, deformability, surface charge mobility, dielectric features, and local capacitance. Thus, different types of particles are simultaneously separated at different points along the channel distance given small variations of properties. The device has shown the ability to separate analytes over a large dynamic range of size, from 20 nm to 1 μm, roughly the size of proteins to the size of cells. In the study of different sized sulfate capped polystyrene particles were shown to be selectively captured as well as concentrating particles from 103 to 106 times. Qualitative capture and manipulation of β-amyloid fibrils were also shown. The results demonstrate the selective focusing ability of the technique; and it may form the foundation for a versatile tool for separating complex mixtures. Combined this work shows promise for future identification of individual organisms from aerosolized protein as well as for applications in biomedical research.
ContributorsStaton, Sarah J. R (Author) / Hayes, Mark A. (Committee member) / Anbar, Ariel D (Committee member) / Shock, Everett (Committee member) / Williams, Peter (Committee member) / Arizona State University (Publisher)
Created2011
Description
In 1999, Geneviève Hasenohr announced the discovery of a fragment of Marguerite Porete's Mirouer des Simples Ames, a work condemned by the Church at the University of Paris in 1310, hidden in a manuscript at the Bibliothèque municipale in Valenciennes. The fragment corresponds with roughly two chapters in the only extant French version of the manuscript (Chantilly, Musée Condé MS F XIV 26), and when compared with other editions of the Mirouer, it appears to be composed in what might have been Marguerite Porete's native dialect. The discovery changed scholars' perceptions of the weight of the various versions and translations - the Chantilly manuscript had been used previously to settle any questions of discrepancy, but now it appears that the Continental Latin and Middle English translations should be the arbiters. This discovery has elevated the Middle English editions, and has made the question of the translator's identity - he is known only by his initials M.N. - and background more imperative to an understanding of why a work with such a dubious history would be translated and harbored by English Carthusians in the century that followed its condemnation. The only candidate suggested for translator of the Mirouer has been Michael Northburgh (d. 1361), the Bishop of London and co-founder of the London Charterhouse, where two of the three remaining copies of the translation were once owned, but the language of the text and Northburgh's own position and interests do not fit this suggestion. My argument is that the content of the book, the method of its translation, its selection as a work for a Latin-illiterate audience, all fit within the interests of a circle of writers based in Yorkshire at the end of the fourteenth century. By beginning among the Yorkshire circle, and widening the search to include writers with a non-traditional contemplative audience, one that exists outside of the cloister - writers like Walter Hilton, the anonymous authors of the Cloud of Unknowing and the Chastising of God's Children, and Nicholas Love - we may have a better chance of locating and understanding the motives of the Middle English translator of the Mirouer.
ContributorsStauffer, Robert F (Author) / Voaden, Rosalynn (Thesis advisor) / Cruse, Markus (Committee member) / Sturges, Robert (Committee member) / Arizona State University (Publisher)
Created2011
Description
The technology expansion seen in the last decade for genomics research has permitted the generation of large-scale data sources pertaining to molecular biological assays, genomics, proteomics, transcriptomics and other modern omics catalogs. New methods to analyze, integrate and visualize these data types are essential to unveil relevant disease mechanisms. Towards these objectives, this research focuses on data integration within two scenarios: (1) transcriptomic, proteomic and functional information and (2) real-time sensor-based measurements motivated by single-cell technology. To assess relationships between protein abundance, transcriptomic and functional data, a nonlinear model was explored at static and temporal levels. The successful integration of these heterogeneous data sources through the stochastic gradient boosted tree approach and its improved predictability are some highlights of this work. Through the development of an innovative validation subroutine based on a permutation approach and the use of external information (i.e., operons), lack of a priori knowledge for undetected proteins was overcome. The integrative methodologies allowed for the identification of undetected proteins for Desulfovibrio vulgaris and Shewanella oneidensis for further biological exploration in laboratories towards finding functional relationships. In an effort to better understand diseases such as cancer at different developmental stages, the Microscale Life Science Center headquartered at the Arizona State University is pursuing single-cell studies by developing novel technologies. This research arranged and applied a statistical framework that tackled the following challenges: random noise, heterogeneous dynamic systems with multiple states, and understanding cell behavior within and across different Barrett's esophageal epithelial cell lines using oxygen consumption curves. These curves were characterized with good empirical fit using nonlinear models with simple structures which allowed extraction of a large number of features. Application of a supervised classification model to these features and the integration of experimental factors allowed for identification of subtle patterns among different cell types visualized through multidimensional scaling. Motivated by the challenges of analyzing real-time measurements, we further explored a unique two-dimensional representation of multiple time series using a wavelet approach which showcased promising results towards less complex approximations. Also, the benefits of external information were explored to improve the image representation.
ContributorsTorres Garcia, Wandaliz (Author) / Meldrum, Deirdre R. (Thesis advisor) / Runger, George C. (Thesis advisor) / Gel, Esma S. (Committee member) / Li, Jing (Committee member) / Zhang, Weiwen (Committee member) / Arizona State University (Publisher)
Created2011
Description
Reduced space is an important theme in the works of the Marquis de Sade including his epic novel The New Justine and his pornographic performance piece Philosophy in the Bedroom including the political/social treatise "Frenchmen, yet another effort is needed if you want to be a Republic". Through out his life Sade attempted to overcome reduction of space with writing. Tragically, his writing often prolonged the reduction of his space by sending him to or keeping him in prison. It is my theory that his violent, pornographic writing style is "une écriture de surjouissance" or "a writing of over-coming". Surjouissance is my theory for Sade's method, based on textual analysis of Sade's main works, that he combines through his syntactic structure, narrative voice, and semantic themes the orgasm of the mind represented by philosophical discourse with the orgasm of the body represented textually by orgiastic scenes and the language of orgasm to reach an ultimate state of complete freedom. In the political pamphlet "Frenchmen yet another effort..."Sade attempts to set this theory of sur-jouissance, or this transcendent state reached through the combination of physical and philosophical orgasm, as the political foundation for a new republic. Does he succeed in creating a viable political formula for a sustainable republic? My argument states absolutely not. His aristocratic elitism narrows his voice. But he does propose the combination of sexual, literary, and intellectual freedoms as a possible polemic against any form of reduced space.
ContributorsSwankie, Ryan James (Author) / Mullet, Isabelle (Thesis advisor) / Canovas, Frédéric (Committee member) / Bahtchevanova, Mariana (Committee member) / Arizona State University (Publisher)
Created2011
Description
The video game graphics pipeline has traditionally rendered the scene using a polygonal approach. Advances in modern graphics hardware now allow the rendering of parametric methods. This thesis explores various smooth surface rendering methods that can be integrated into the video game graphics engine. Moving over to parametric or smooth surfaces from the polygonal domain has its share of issues and there is an inherent need to address various rendering bottlenecks that could hamper such a move. The game engine needs to choose an appropriate method based on in-game characteristics of the objects; character and animated objects need more sophisticated methods whereas static objects could use simpler techniques. Scaling the polygon count over various hardware platforms becomes an important factor. Much control is needed over the tessellation levels, either imposed by the hardware limitations or by the application, to be able to adaptively render the mesh without significant loss in performance. This thesis explores several methods that would help game engine developers in making correct design choices by optimally balancing the trade-offs while rendering the scene using smooth surfaces. It proposes a novel technique for adaptive tessellation of triangular meshes that vastly improves speed and tessellation count. It develops an approximate method for rendering Loop subdivision surfaces on tessellation enabled hardware. A taxonomy and evaluation of the methods is provided and a unified rendering system that provides automatic level of detail by switching between the methods is proposed.
ContributorsAmresh, Ashish (Author) / Farin, Gerlad (Thesis advisor) / Razdan, Anshuman (Thesis advisor) / Wonka, Peter (Committee member) / Hansford, Dianne (Committee member) / Arizona State University (Publisher)
Created2011
Description
Most research on the experience of young women in student service roles in higher education is focused on a reflection of the early career experiences of mid and senior level professionals. Young women enter the field with a set of expectations about the work and their early career experiences need to be uncovered in order to better understand what they expect from their roles in student services. This study focused on the experience of young women in student services and the dynamics they identify as being significant to their work experience. Six women in their mid-twenties working in student service roles participated in two dialogic interviews regarding their work experience. Findings from these women's stories suggest that women are aware of internal and external dynamics that shaped their work experience, and are engaged in their journey toward Self-Authorship along intrapersonal and interpersonal dimensions. Specifically, the women actively chose their career path, looked for opportunities to develop their professional cache, and were impacted by their relationships with their supervisors and colleagues. The women are interested in their professional development in student services in higher education and are active in shaping the experience to meet their expectations. The findings suggest that to understand the experience of young women in student service roles in higher education, women should be asked to share their stories on their early career experiences, including interactions with supervisors and other professional colleagues. By representing these voices in the dialogue on the experience of young women in student service roles, the dynamics that shaped those experiences can be better understood.
ContributorsBeech, Rachel A (Author) / Ewing, Kris M (Thesis advisor) / Calleroz White, Mistalene (Committee member) / Sullivan, Linda (Committee member) / Arizona State University (Publisher)
Created2011
Description
Masculinity has been increasingly recognized as a critical and relatively unexplored area of inquiry in anthropological gender studies. This project seeks to expand anthropological research on masculinity to contemporary American society. Using the case study of a male-centered popular new sport, Mixed Martial Arts (also known as cagefighting) this project integrates theories of embodiment and feminist perspectives to explore how masculinity and masculine hegemony are shaped, contested, and perpetuated in the United States. Using a multi-level framework this project explores: 1) How is masculinity experienced and expressed by Mixed Martial Arts fighters as a form of self-identity? How do their bodies play a role in constructing masculinity? 2) What are the pervasive forms of masculinity associated with Mixed Martial Arts (MMA)? Are they truly representative of the sport? 3) Can these pervasive forms of masculinity be seen as hegemonic? How would hegemony operate in relation to individual experience? Using multiple methods to capture multiple points of view was critical to thoroughly examining the complex notion of masculinity. This study employed participant observation, semi-structured interviews, focus groups, surveys, photo elicitation, and media content analysis, as each presented particular benefits and allowed for the development a more well-rounded understanding of masculinity within the realm of MMA. This study also situates the rise of MMA and its representations of masculinity within the greater perspective of contemporary American society. By doing so reveals how ideologies of prescribed masculinity do not arise out of a vacuum but in relation to particular economic, social and political contexts. An emphasis of this study was to examine the daily lives of MMA fighters to understand how their participation in what may be regarded as a hypermasculine activity affects their own perceptions of masculinity. In looking at how masculinity is embodied, the gaps and often contradictions between representation and individual experiences are revealed. Ultimately, the goal of this research is to contribute to a better understanding of masculinity as both an embodied and relational construct.
ContributorsHolthuysen, Jaime (Author) / Hjorleifur Jonsson (Thesis advisor) / Tsuda, Takeyuki (Committee member) / Ballestero-Salaverry, Andrea (Committee member) / Arizona State University (Publisher)
Created2011
Description
Since the Convention on Biological Diversity was established in 1992, more importance has been given to the conservation of genetic resources in the international community. In 2001, the International Treaty on Plant Genetic Resources for Food and Agriculture (PGRFA) focused on conserving plant genetic resources, including crop wild relatives (CWR). Some of these genetic resources hold desirable traits--such as transfer of plant disease resistance, improvement of nutritional content, or increased resistance to climate change--that can improve commercial crops. For many years, ex situex situ conservation was the prevalent form of protecting plant genetic resources. However, after PGRFA was published in 1998, in situ techniques have increasingly been applied to conserve wild relatives and enhance domesticated crops.In situ techniques are preferred when possible, since they allow for continued evolution of traits through natural selection, and viability of seed stock through continuous germination and regeneration. In my research, I identified regions in Bolivia and rated them according to their potential for successful programs of iin situ conservation of wild crop relatives. In particular, I analyzed areas according to the following criteria: a) The prevalence of CWRs. b) The impacts of climate change, land use change, population growth, and economic development on the continued viability of CWRs in an area. c) The socio-political and economic conditions that might impede or facilitate successful conservation programs and outcomes. This work focuses on three genera of particular importance in Bolivia: Peanut (Arachis spp.), Potato (Solanum spp.) and Quinoa (Chenopodium spp.). I analyzed the above factors for each municipality in Bolivia (the smallest scale for which appropriate data were available). The results indicate which municipalities are most likely to successfully engage in CWR conservation projects. Finally, I present guidelines for the creation of conservation projects that pinpoint some of the potential risks and difficulties with in situ conservation programs in Bolivia and more generally.
ContributorsGonzalez-Paredes, Cecilia (Author) / Kinzig, Ann (Thesis advisor) / Aggarwal, Rimjhim (Committee member) / Chhetri, Netra (Committee member) / Arizona State University (Publisher)
Created2011
Description
Though some scholars have written about place and history, few have pursued the use of place theory in length in relation to the connections between race, religion, and national identity. Using the writings in the United States and Louisiana in the years surrounding the Louisiana Purchase, I explore place-making and othering processes. U.S. leaders influenced by the Second Great Awakening viewed New Orleans as un-American in its religion and seemingly ambiguous race relations. New Orleanian Catholics viewed the U.S. as an aggressively Protestant place that threatened the stability of the Catholic Church in the Louisiana Territory. Both Americans and New Orleanians constructed the place identities of the other in relation to events in Europe and the Caribbean, demonstrating that places are constructed in relation to one another. In order to elucidate these dynamics, I draw on place theory, literary analysis, and historical anthropology in analyzing the letters of W.C.C. Claiborne, the first U.S. governor of the Louisiana Territory, in conjunction with sermons of prominent Protestant ministers Samuel Hopkins and Jedidiah Morse, a letter written by Ursuline nun Sister Marie Therese de St. Xavior Farjon to Thomas Jefferson, and George Washington Cable's Reconstruction era novel The Grandissimes. All of these parties used the notion of place to create social fact that was bound up with debates about race and anti-Catholic sentiments. Furthermore, their treatments of place demonstrate concerns for creating, or resisting absorption by, a New Republic that was white and Protestant. Place theory proves useful in clarifying how Americans and New Orleanians viewed the Louisiana Purchase as well as the legacy of those ideas. It demonstrates the ways in which the U.S. defined itself in contradistinction to religious others. Limitations arise, however, depending on the types of sources historians use. While official government letters reveal much when put into the context of the trends in American religion at the turn of the nineteenth century, they are not as clearly illuminating as journals and novels. In these genres, authors provide richer detail from which historians can try to reconstruct senses of place.
ContributorsBilinsky, Stephanie (Author) / Fessenden, Tracy (Thesis advisor) / Moore, Moses (Committee member) / Feldhaus, Anne (Committee member) / Arizona State University (Publisher)
Created2011
Description
The term "White flight" and its effects are well documented in large urban city centers. However, few studies consider the same effects on smaller American communities. This case study investigates Lexington, Nebraska, a rural community of approximately 10,000 citizens, that has experienced a population influx of minorities in the last 25 years. The population shift has increased the representation of Hispanic, Asian, and now Somali students in the Lexington Public School system, which, in turn, has been accompanied by a dramatic decrease in White, Anglo students. This study attempts to identify and describe the reasons for the exodus of White students from the public school setting. Possible reasons that might explain the decreases in White student enrollment may include overcrowding in schools, unsafe school environments, and/or less one-on-one attention with classroom teachers.
ContributorsFarnsworth, Joseph C (Author) / Glass, Gene V (Thesis advisor) / Danzig, Arnold (Committee member) / Davey, Lynn (Committee member) / Arizona State University (Publisher)
Created2011