Full metadata
Title
Towards single molecule DNA sequencing
Description
Single molecule DNA Sequencing technology has been a hot research topic in the recent decades because it holds the promise to sequence a human genome in a fast and affordable way, which will eventually make personalized medicine possible. Single molecule differentiation and DNA translocation control are the two main challenges in all single molecule DNA sequencing methods. In this thesis, I will first introduce DNA sequencing technology development and its application, and then explain the performance and limitation of prior art in detail. Following that, I will show a single molecule DNA base differentiation result obtained in recognition tunneling experiments. Furthermore, I will explain the assembly of a nanofluidic platform for single strand DNA translocation, which holds the promised to be integrated into a single molecule DNA sequencing instrument for DNA translocation control. Taken together, my dissertation research demonstrated the potential of using recognition tunneling techniques to serve as a general readout system for single molecule DNA sequencing application.
Date Created
2013
Contributors
- Liu, Hao (Author)
- Lindsay, Stuart M (Committee member)
- Yan, Hao (Committee member)
- Levitus, Marcia (Committee member)
- Arizona State University (Publisher)
Topical Subject
Resource Type
Extent
xii, 75 p. : ill. (some col.)
Language
eng
Copyright Statement
In Copyright
Primary Member of
Peer-reviewed
No
Open Access
No
Handle
https://hdl.handle.net/2286/R.I.17827
Statement of Responsibility
by Hao Liu
Description Source
Retrieved on Nov. 12, 2013
Level of coding
full
Note
thesis
Partial requirement for: Ph.D., Arizona State University, 2013
bibliography
Includes bibliographical references (p. 66-75)
Field of study: Biochemistry
System Created
- 2013-07-12 06:19:18
System Modified
- 2021-08-30 01:42:14
- 3 years 3 months ago
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