Full metadata
Title
Structure investigations of membrane protein OEP16
Description
Membrane protein structure is continuing to be a topic of interest across the scientific community. However, high resolution structural data of these proteins is difficult to obtain. The amino acid transport protein, Outer Envelope Protein, 16kDa (OEP16) is a transmembrane protein channel that allows the passive diffusion of amino acids across the outer chloroplast membrane, and is used as a model protein in order to establish methods that ultimately reveal structural details about membrane proteins using nuclear magnetic resonance (NMR) spectroscopy. Methods include recombinant expression of isotope enriched inclusion bodies, purification and reconstitution in detergent micelles, and pre-characterization techniques including circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), and high pressure liquid chromatography (HPLC). High resolution NMR spectroscopy was able to assign 99% of the amide backbone and the chemical shifts provided detailed secondary structure of OEP16 on a per residue basis using the software TALOS+. Relaxation studies explored the intramolecular dynamics of OEP16 and results strongly support the resonance assignments. Successful titration studies were able to locate residues important for amino acid binding for import into the chloroplast as well as provide information on how the transmembrane helices of OEP16 are packed together. For the first time there is experimental evidence that can assign the location of secondary structure in OEP16 and creates a foundation for a future three dimensional structure.
Date Created
2012
Contributors
- Zook, James Duncan (Author)
- Fromme, Petra (Thesis advisor)
- Chen, Julian (Committee member)
- Wachter, Rebekka (Committee member)
- Arizona State University (Publisher)
Topical Subject
Resource Type
Extent
xi, 155 p. : ill. (some col.)
Language
Copyright Statement
In Copyright
Primary Member of
Peer-reviewed
No
Open Access
No
Handle
https://hdl.handle.net/2286/R.I.15008
Statement of Responsibility
by James Duncan Zook
Description Source
Retrieved on May 2, 2013
Level of coding
full
Note
Vita
Partial requirement for: Ph.D., Arizona State University, 2012
Note type
thesis
Includes bibliographical references (p. 141-149)
Note type
bibliography
Field of study: Biochemistry
System Created
- 2012-08-24 06:27:56
System Modified
- 2021-08-30 01:46:10
- 3 years ago
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