Full metadata
Title
Analyzing rat sciatic nerve fibers under various electrical stimuli
Description
Abstract Modern imaging techniques for sciatic nerves often use imaging techniques that can clearly find myelinated axons (Group A and Group B and analyze their properties, but have trouble with the more numerous Remak Fibers (Group C). In this paper, Group A and B fibers are analyzed while also analyzing Remak fibers using osmium tetroxide staining and imaging with the help of transmission electron microscopy. Using this method, nerves had various electrical stimuli attached to them and were analyzed as such. They were analyzed with a cuff electrode attached, a stimulator attached, and both, with images taken at the center of the nerve and the ends of them. The number and area taken by the Remak fibers were analyzed, along with the g-ratios of the Group A and B fibers. These were analyzed to help deduce the overall health of the fibers along with vacuolization, and mitochondria available. While some important information was gained from this evaluation, further testing has to be done to improve the myelin detection system, along with analyzing the proper and necessary Remak fibers and the role they play. The research tries to thoroughly look at the necessary material and find a way to use it as a guide to further experimentation with electrical stimuli, and notes the differences found within and without various groups, various points of observation, and various stimuli as a whole. Nevertheless, this research allows a strong look into the benefits of transmission electron microscopy and the ability to assess electrical stimulation from these points.
Date Created
2018-05
Contributors
- Nambiar, Karthik (Author)
- Muthuswamy, Jitendran (Thesis director)
- Towe, Bruce (Committee member)
- Harrington Bioengineering Program (Contributor)
- School of Mathematical and Statistical Sciences (Contributor)
- Barrett, The Honors College (Contributor)
Topical Subject
Resource Type
Extent
14 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Series
Academic Year 2017-2018
Handle
https://hdl.handle.net/2286/R.I.48265
Level of coding
minimal
Cataloging Standards
System Created
- 2018-04-21 12:24:12
System Modified
- 2021-08-11 04:09:57
- 3 years 2 months ago
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