Full metadata

Title
Double-flow Focused Liquid Injector for Efficient Serial Femtosecond Crystallography
Description

Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

Date Created
2017-03-16
Contributors
Resource Type
Text
Extent
10 pages
Language
eng
Copyright Statement
In Copyright
Reuse Permissions
Attribution
Primary Member of
ASU Regents' Professors Open Access Works
Identifier
Digital object identifier: 10.1038/srep44628
International standard serial number
2045-2322
Peer-reviewed
No
Open Access
No
Series
SCIENTIFIC REPORTS
Handle
https://hdl.handle.net/2286/R.I.43943
Preferred Citation

Oberthuer, D., Knoška, J., Wiedorn, M. O., Beyerlein, K. R., Bushnell, D. A., Kovaleva, E. G., . . . Bajt, S. (2017). Double-flow focused liquid injector for efficient serial femtosecond crystallography. Scientific Reports, 7, 44628. doi:10.1038/srep44628

Level of coding
minimal
Cataloging Standards
asu1
Note
The final version of this article, as published in Scientific Reports, can be viewed online at: https://www.nature.com/articles/srep44628
System Created
  • 2017-06-01 01:03:28
System Modified
  • 2021-09-07 05:41:47
    •     
  • 3 years 2 months ago
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