Full metadata
Title
The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography
Description
Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 Å resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 Å resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.
Date Created
2016-10-19
Contributors
- Edlund, Petra (Author)
- Takala, Heikki (Author)
- Claesson, Elin (Author)
- Henry, Leocadie (Author)
- Dods, Robert (Author)
- Lehtivuori, Heli (Author)
- Panman, Matthijs (Author)
- Pande, Kanupriya (Author)
- White, Thomas (Author)
- Nakane, Takanori (Author)
- Berntsson, Oskar (Author)
- Gustavsson, Emil (Author)
- Bath, Petra (Author)
- Modi, Vaibhav (Author)
- Roy Chowdhury, Shatabdi (Author)
- Zook, James (Author)
- Berntsen, Peter (Author)
- Pandey, Suraj (Author)
- Poudyal, Ishwor (Author)
- Tenboer, Jason (Author)
- Kupitz, Christopher (Author)
- Barty, Anton (Author)
- Fromme, Petra (Author)
- Koralek, Jake D. (Author)
- Tanaka, Tomoyuki (Author)
- Spence, John (Author)
- Liang, Mengning (Author)
- Hunter, Mark S. (Author)
- Boutet, Sebastien (Author)
- Nango, Eriko (Author)
- Moffat, Keith (Author)
- Groenhof, Gerrit (Author)
- Ihalainen, Janne (Author)
- Stojkovic, Emina A. (Author)
- Schmidt, Marius (Author)
- Westenhoff, Sebastian (Author)
- College of Liberal Arts and Sciences (Contributor)
- School of Molecular Sciences (Contributor)
- Biodesign Institute (Contributor)
- Applied Structural Discovery (Contributor)
- Department of Physics (Contributor)
Resource Type
Extent
9 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Identifier
Digital object identifier: 10.1038/srep35279
Identifier Type
International standard serial number
Identifier Value
2045-2322
Series
SCIENTIFIC REPORTS
Handle
https://hdl.handle.net/2286/R.I.44380
Preferred Citation
Edlund, P., Takala, H., Claesson, E., Henry, L., Dods, R., Lehtivuori, H., . . . Westenhoff, S. (2016). The room temperature crystal structure of a bacterial phytochrome determined by serial femtosecond crystallography. Scientific Reports, 6(1). doi:10.1038/srep35279
Level of coding
minimal
Cataloging Standards
Note
The final version of this article, as published in Scientific Reports, can be viewed online at: https://www.nature.com/articles/srep35279
System Created
- 2017-06-05 01:23:36
System Modified
- 2021-08-16 02:23:30
- 3 years 2 months ago
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