Full metadata
Title
Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
Description
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65–70 nm, which is considerably smaller than the previously reported ~600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.
Date Created
2017-06-27
Contributors
- Reddy, Hemanth K. N. (Author)
- Yoon, Chun Hong (Author)
- Aquila, Andrew (Author)
- Awel, Salah (Author)
- Ayyer, Kartik (Author)
- Barty, Anton (Author)
- Berntsen, Peter (Author)
- Bielecki, Johan (Author)
- Bobkov, Sergey (Author)
- Bucher, Maximilian (Author)
- Carini, Gabriella A. (Author)
- Carron, Sebastian (Author)
- Chapman, Henry (Author)
- Daurer, Benedikt (Author)
- DeMirci, Hasan (Author)
- Ekeberg, Tomas (Author)
- Fromme, Petra (Author)
- Hajdu, Janos (Author)
- Hanke, Max Felix (Author)
- Hart, Philip (Author)
- Hogue, Brenda (Author)
- Hasseinizadeh, Ahmad (Author)
- Kim, Yoonhee (Author)
- Kirian, Richard (Author)
- Kurta, Ruslan P. (Author)
- Larsson, Daniel S. D. (Author)
- Loh, N. Duane (Author)
- Maia, Filipe R. N. C. (Author)
- Mancuso, Adrian P. (Author)
- Muhlig, Kerstin (Author)
- Munke, Anna (Author)
- Nam, Daewoong (Author)
- Nettelblad, Carl (Author)
- Ourmazd, Abbas (Author)
- Rose, Max (Author)
- Schwander, Peter (Author)
- Seibert, Marvin (Author)
- Sellberg, Jonas A. (Author)
- Song, Changyong (Author)
- Spence, John (Author)
- Svenda, Martin (Author)
- van der Schot, Gijs (Author)
- Vartanyants, Ivan A. (Author)
- Williams, Garth J. (Author)
- Xavier, P. Lourdu (Author)
- ASU Biodesign Center Immunotherapy, Vaccines and Virotherapy (Contributor)
- Biodesign Institute (Contributor)
- Applied Structural Discovery (Contributor)
- College of Liberal Arts and Sciences (Contributor)
- School of Molecular Sciences (Contributor)
- School of Life Sciences (Contributor)
- Department of Physics (Contributor)
Resource Type
Extent
9 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Identifier
Digital object identifier: 10.1038/sdata.2017.79
Identifier Type
International standard serial number
Identifier Value
2052-4463
Series
SCIENTIFIC DATA
Handle
https://hdl.handle.net/2286/R.I.45082
Preferred Citation
Reddy, H. K., Yoon, C. H., Aquila, A., Awel, S., Ayyer, K., Barty, A., . . . Xavier, P. L. (2017). Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source. Scientific Data, 4, 170079. doi:10.1038/sdata.2017.79
Level of coding
minimal
Cataloging Standards
Note
The final version of this article, as published in Scientific Data, can be viewed online at: http://www.nature.com/articles/sdata201779
System Created
- 2017-08-10 12:28:01
System Modified
- 2021-08-16 02:23:30
- 3 years 2 months ago
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