Full metadata
Title
Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Description
We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.
Date Created
2016-09-20
Contributors
- Ishchenko, Andrii (Author)
- Cherezov, Vadim (Author)
- Liu, Wei (Author)
- College of Liberal Arts and Sciences (Contributor)
Resource Type
Extent
8 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Identifier
Digital object identifier: 10.3791/54463
Identifier Type
International standard serial number
Identifier Value
1940-087X
Peer-reviewed
No
Open Access
No
Series
JOURNAL OF VISUALIZED EXPERIMENTS
Handle
https://hdl.handle.net/2286/R.I.45316
Preferred Citation
Ishchenko, A., Cherezov, V., & Liu, W. (2016). Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography. Journal of Visualized Experiments, (115). doi:10.3791/54463
Level of coding
minimal
Cataloging Standards
Note
The final version of this article, as published in Journal of Visualized Experiments, can be viewed online at: https://www.jove.com/video/54463/preparation-delivery-protein-microcrystals-lipidic-cubic-phase-for
System Created
- 2017-08-28 04:47:59
System Modified
- 2021-12-08 05:01:02
- 2 years 11 months ago
Additional Formats